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Lmx1b is required for the glutamatergic fates of a subset of spinal cord neurons

Hilinski, William C.; Bostrom, Jonathan R.; England, Samantha J.; Juarez-Morales, Jose L.; Jager, Sarah de; Armant, Olivier; Legradi, Jessica; Straehle, Uwe; Link, Brian A.; Lewis, Katharine E.

Background: Alterations in neurotransmitter phenotypes of specific neurons can cause imbalances in excitation and inhibition in the central nervous system (CNS), leading to diseases. Therefore, the correct specification and maintenance of neurotransmitter phenotypes is vital. As with other neuronal properties, neurotransmitter phenotypes are often specified and maintained by particular transcription factors. However, the specific molecular mechanisms and transcription factors that regulate neurotransmitter phenotypes remain largely unknown.
Methods: In this paper we use single mutant, double mutant and transgenic zebrafish embryos to elucidate the functions of Lmx1ba and Lmx1bb in the regulation of spinal cord interneuron neurotransmitter phenotypes.
Results: We demonstrate that lmx1ba and lmx1bb are both expressed in zebrafish spinal cord and that lmx1bb is expressed by both V0v cells and dI5 cells. Our functional analyses demonstrate that these transcription factors are not required for neurotransmitter fate specification at early stages of development, but that in embryos with at least two lmx1ba and/or lmx1bb mutant alleles there is a reduced number of excitatory (glutamatergic) spinal interneurons at later stages of development. ... mehr

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DOI: 10.5445/IR/1000059890
DOI: 10.1186/s13064-016-0070-1
Zitationen: 6
Zitationen: 9
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Toxikologie und Genetik (ITG)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2016
Sprache Englisch
Identifikator ISSN: 1749-8104
KITopen-ID: 1000059890
HGF-Programm 47.01.01 (POF III, LK 01) Biol.Netzwerke u.Synth.Regulat. ITG+ITC
Erschienen in Neural Development
Verlag BioMed Central (BMC)
Band 11
Heft 1
Seiten Art.Nr.:16
Schlagwörter Spinal cord, Interneuron, Zebrafish, Lmx1b, Excitatory, Neurotransmitter, CNS, Transcription factor, V0v, dI5
Nachgewiesen in Web of Science
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