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TGF-beta 1 Is Present at High Levels in Wound Fluid from Breast Cancer Patients Immediately Post-Surgery, and Is Not Increased by Intraoperative Radiation Therapy (IORT)

Scherer, Sandra D. 1; Bauer, Jochen 1; Schmaus, Anja 1; Neumaier, Christian; Herskind, Carsten; Veldwijk, Marlon R.; Wenz, Frederik; Sleeman, Jonathan P. 1
1 Institut für Toxikologie und Genetik (ITG), Karlsruher Institut für Technologie (KIT)

Abstract:

In patients with low-risk breast cancer, intraoperative radiotherapy (IORT) during breastconserving surgery is a novel and convenient treatment option for delivering a single high dose of irradiation directly to the tumour bed. However, edema and fibrosis can develop after surgery and radiotherapy, which can subsequently impair quality of life. TGF- β is a strong inducer of the extracellular matrix component hyaluronan (HA). TGF-β expression and HA metabolism can be modulated by irradiation experimentally, and are involved in edema and fibrosis. We therefore hypothesized that IORT may regulate these factors. Wound fluid (WF) draining from breast lumpectomy sites was collected and levels of TGF-β1 and HA were determined by ELISA. Proliferation and marker expression was analyzed in primary lymphatic endothelial cells (LECs) treated with recombinant TGF-β or WF. Our results show that IORT does not change TGF-β1 or HA levels in wound fluid draining from breast lumpectomy sites, and does not lead to accumulation of sHA oligosaccharides. Nevertheless, concentrations of TGF-β1 were high in WF from patients regardless of IORT, at concentrations well above those associated with fibrosis and the suppression of LEC identity. ... mehr


Verlagsausgabe §
DOI: 10.5445/IR/1000060078
Veröffentlicht am 18.01.2018
Originalveröffentlichung
DOI: 10.1371/journal.pone.0162221
Scopus
Zitationen: 9
Web of Science
Zitationen: 9
Dimensions
Zitationen: 11
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Toxikologie und Genetik (ITG)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2016
Sprache Englisch
Identifikator ISSN: 1932-6203
urn:nbn:de:swb:90-600785
KITopen-ID: 1000060078
HGF-Programm 47.01.01 (POF III, LK 01) Biol.Netzwerke u.Synth.Regulat. ITG+ITC
Erschienen in PLoS one
Verlag Public Library of Science (PLoS)
Band 11
Heft 9
Seiten Art. Nr.: e0162221
Vorab online veröffentlicht am 02.09.2016
Nachgewiesen in Dimensions
Scopus
Web of Science
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