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DOI: 10.5445/IR/1000060219
DOI: 10.1515/cdbme-2016-0052

Semi-automated detection of fractional shortening in zebrafish embryo heart videos

Nasrat, Sara; Marcato, Daniel; Hirth, Sofia; Reischl, Markus; Pylatiuk, Christian

Abstract (englisch):
Quantifying cardiac functions in model organisms like embryonic zebrafish is of high importance in small molecule screens for new therapeutic compounds. One relevant cardiac parameter is the fractional shortening (FS). A method for semi-automatic quantification of FS in video recordings of zebrafish embryo hearts is presented. The software provides automated visual information about the end-systolic and end-diastolic stages of the heart by displaying corresponding colored lines into a Motion-mode display. After manually marking the ventricle diameters in frames of end-systolic and end-diastolic stages, the FS is calculated. The software was evaluated by comparing the results of the determination of FS with results obtained from another established method. Correlations of 0.96 < r < 0.99 between the two methods were found indicating that the new software provides comparable results for the determination of the FS.

Zugehörige Institution(en) am KIT Institut für Toxikologie und Genetik (ITG)
Institut für Angewandte Informatik (IAI)
Publikationstyp Zeitschriftenaufsatz
Jahr 2016
Sprache Englisch
Identifikator ISSN: 2364-5504
URN: urn:nbn:de:swb:90-602199
KITopen-ID: 1000060219
HGF-Programm 47.01.02 (POF III, LK 01)
Erschienen in Current directions in biomedical engineering
Band 2
Heft 1
Seiten 233-236
Schlagworte fractional shortening, heart, semi-automatic quantification, zebrafish
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