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Semi-automated detection of fractional shortening in zebrafish embryo heart videos

Nasrat, Sara; Marcato, Daniel; Hirth, Sofia; Reischl, Markus; Pylatiuk, Christian

Abstract (englisch):
Quantifying cardiac functions in model organisms like embryonic zebrafish is of high importance in small molecule screens for new therapeutic compounds. One relevant cardiac parameter is the fractional shortening (FS). A method for semi-automatic quantification of FS in video recordings of zebrafish embryo hearts is presented. The software provides automated visual information about the end-systolic and end-diastolic stages of the heart by displaying corresponding colored lines into a Motion-mode display. After manually marking the ventricle diameters in frames of end-systolic and end-diastolic stages, the FS is calculated. The software was evaluated by comparing the results of the determination of FS with results obtained from another established method. Correlations of 0.96 < r < 0.99 between the two methods were found indicating that the new software provides comparable results for the determination of the FS.

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Volltext §
DOI: 10.5445/IR/1000060219
DOI: 10.1515/cdbme-2016-0052
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Toxikologie und Genetik (ITG)
Institut für Angewandte Informatik (IAI)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2016
Sprache Englisch
Identifikator ISSN: 2364-5504
KITopen-ID: 1000060219
HGF-Programm 47.01.02 (POF III, LK 01)
Biol.Netzwerke u.Synth.Regulat. IAI
Erschienen in Current directions in biomedical engineering
Band 2
Heft 1
Seiten 233-236
Schlagwörter fractional shortening, heart, semi-automatic quantification, zebrafish
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