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The RTR Complex Partner RMI2 and the DNA Helicase RTEL1 Are Both Independently Involved in Preserving the Stability of 45S rDNA Repeats in Arabidopsis thaliana

Röhrig, S. 1; Schröpfer, S. 1; Knoll, A. 1; Puchta, H. 1
1 Karlsruher Institut für Technologie (KIT)

Abstract (englisch):

The stability of repetitive sequences in complex eukaryotic genomes is safeguarded by factors suppressing homologues recombination. Prominent in this is the role of the RTR complex. In plants, it consists of the RecQ helicase RECQ4A, the topoisomerase TOP3α and RMI1. Like mammals, but not yeast, plants harbor an additional complex partner, RMI2. Here, we demonstrate that, in Arabidopsis thaliana, RMI2 is involved in the repair of aberrant replication intermediates in root meristems as well as in intrastrand crosslink repair. In both instances, RMI2 is involved independently of the DNA helicase RTEL1. Surprisingly, simultaneous loss of RMI2 and RTEL1 leads to loss of male fertility. As both the RTR complex and RTEL1 are involved in suppression of homologous recombination (HR), we tested the efficiency of HR in the double mutant rmi2-2 rtel1-1 and found a synergistic enhancement (80-fold). Searching for natural target sequences we found that RTEL1 is required for stabilizing 45S rDNA repeats. In the double mutant with rmi2-2 the number of 45S rDNA repeats is further decreased sustaining independent roles of both factors in this process. ... mehr

Volltext §
DOI: 10.5445/IR/1000062183
DOI: 10.1371/journal.pgen.1006394
Zitationen: 24
Web of Science
Zitationen: 23
Zitationen: 26
Cover der Publikation
Zugehörige Institution(en) am KIT Fakultät für Chemie und Biowissenschaften – Botanisches Institut und Botanischer Garten (BOTANIK)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2016
Sprache Englisch
Identifikator ISSN: 1553-7390, 1553-7404
KITopen-ID: 1000062183
Erschienen in PLoS Genetics
Verlag Public Library of Science (PLoS)
Band 12
Heft 10
Seiten e1006394
Bemerkung zur Veröffentlichung Gefördert durch den KIT-Publikationsfonds
Nachgewiesen in Dimensions
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