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Substrate specificity and function of acetylpolyamine amidohydrolases from Pseudomonas aeruginosa

Krämer, Andreas; Herzer, Jan; Overhage, Jörg; Meyer-Almes, Franz-Josef

Background: Pseudomonas aeruginosa, a Gram-negative, aerobic coccobacillus bacterium is an opportunistic human pathogen and worldwide the fourth most common cause of hospital-acquired infections which are often high mortality such as ventilator-associated pneumoniae. The polyamine metabolism of P. aeruginosa and particularly the deacetylation of acetylpolyamines has been little studied up to now. Results with other bacterial pathogens e.g., Y. pestis suggest that polyamines may be involved in the formation of biofilms or confer resistance against certain antibiotics.
Results: To elucidate the role of acetylpolyamines and their enzymatic deacetylation in more detail, all three putative acetylpolyamine amidohydrolases (APAHs) from P. aeruginosa have been expressed in enzymatic active form. The APAHs PA0321 and PA1409 are shown to be true polyamine deacetylases, whereas PA3774 is not able to deacetylate acetylated polyamines. Every APAH can hydrolyze trifluoroacetylated lysine-derivatives, but only PA1409 and much more efficiently PA3774 can also process the plain acetylated lysine substrate. P. aeruginosa is able to utilize acetylcadaverine and acetylputrescine as a carbon source under glucose starvation. ... mehr

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DOI: 10.5445/IR/1000062791
DOI: 10.1186/s12858-016-0063-z
Zitationen: 9
Zitationen: 9
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Funktionelle Grenzflächen (IFG)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2016
Sprache Englisch
Identifikator ISSN: 1471-2091
KITopen-ID: 1000062791
HGF-Programm 47.02.06 (POF III, LK 01) Zellpopul.auf Biofunk.Oberflächen IFG
Erschienen in BMC biochemistry
Verlag BioMed Central (BMC)
Band 17
Heft 1
Seiten Art.Nr.: 63
Schlagwörter Acetylpolyamine amidohydrolases, Pseudomonas aeruginosa, Substrate specificity, Acetylpolyamines, Polyamine metabolism
Nachgewiesen in Dimensions
Web of Science
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