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Droplet Microarray Based on Superhydrophobic-Superhydrophilic Patterns for Single Cell Analysis

Jogia, Gabriella; Tronser, Tina; Popova, Anna; Levkin, Pavel ORCID iD icon

Abstract (englisch):

Single-cell analysis provides fundamental information on individual cell response to different environmental cues and is a growing interest in cancer and stem cell research. However, current existing methods are still facing challenges in performing such analysis in a high-throughput manner whilst being cost-effective. Here we established the Droplet Microarray (DMA) as a miniaturized screening platform for high-throughput single-cell analysis. Using the method of limited dilution and varying cell density and seeding time, we optimized the distribution of single cells on the DMA. We established culturing conditions for single cells in individual droplets on DMA obtaining the survival of nearly 100% of single cells and doubling time of single cells comparable with that of cells cultured in bulk cell population using conventional methods. Our results demonstrate that the DMA is a suitable platform for single-cell analysis, which carries a number of advantages compared with existing technologies allowing for treatment, staining and spot-to-spot analysis of single cells over time using conventional analysis methods such as microscopy. View Full-Text

Volltext §
DOI: 10.5445/IR/1000064156
DOI: 10.3390/microarrays5040028
Zitationen: 30
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Toxikologie und Genetik (ITG)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2016
Sprache Englisch
Identifikator ISSN: 2076-3905
KITopen-ID: 1000064156
HGF-Programm 47.01.01 (POF III, LK 01) Biol.Netzwerke u.Synth.Regulat. ITG+ITC
Erschienen in Microarrays
Verlag MDPI
Band 5
Heft 4
Seiten 28
Schlagwörter Droplet Microarray, superhydrophobic-superhydrophilic patterning, single-cell analysis, high-throughput screening, screening
Nachgewiesen in Dimensions
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