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Cadherin-11 localizes to focal adhesions and promotes cell-substrate adhesion

Langhe, Rahul P.; Gudzenko, Tetyana; Bachmann, Michael; Becker, Sarah F.; Gonnermann, Carina; Winter, Claudia; Abbruzzese, Genevieve; Alfandari, Dominique; Kratzer, Marie-Claire; Franz, Clemens M.; Kashef, Jubin

Cadherin receptors have a well-established role in cell–cell adhesion, cell polarization and differentiation. However, some cadherins also promote cell and tissue movement during embryonic development and tumour progression. In particular, cadherin-11 is upregulated during tumour and inflammatory cell invasion, but the mechanisms underlying cadherin-11 stimulated cell migration are still incompletely understood. Here, we show that cadherin-11 localizes to focal adhesions and promotes adhesion to fibronectin in Xenopus neural crest, a highly migratory embryonic cell population. Transfected cadherin-11 also localizes to focal adhesions in different mammalian cell lines, while endogenous cadherin-11 shows focal adhesion localization in primary human fibroblasts. In focal adhesions, cadherin-11 co-localizes with β1-integrin and paxillin and physically interacts with the fibronectin-binding proteoglycan syndecan-4. Adhesion to fibronectin mediated by cadherin-11/syndecan-4 complexes requires both the extracellular domain of syndecan-4, and the transmembrane and cytoplasmic domains of cadherin-11. These results reveal an unexpected role o ... mehr

Zugehörige Institution(en) am KIT Center for Functional Nanostructures (CFN)
ANKA - die Synchrotronstrahlungsquelle am KIT (ANKA)
Laboratorium für Applikationen der Synchrotronstrahlung (LAS)
Zoologisches Institut (ZOO)
Institut für Photonenforschung und Synchrotronstrahlung (IPS)
Publikationstyp Zeitschriftenaufsatz
Jahr 2016
Sprache Englisch
Identifikator DOI: 10.1038/ncomms10909
ISSN: 2041-1723
URN: urn:nbn:de:swb:90-641652
KITopen ID: 1000064165
Erschienen in Nature Communications
Band 7
Seiten Art.Nr.: 10909
Lizenz CC BY 4.0: Creative Commons Namensnennung 4.0 International
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