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DNA-DNA kissing complexes as a new tool for the assembly of DNA nanostructures

Barth, Anna; Kobbe, Daniela; Focke, Manfred

Kissing-loop annealing of nucleic acids occurs in nature in several viruses and in prokaryotic replication, among other circumstances. Nucleobases of two nucleic acid strands (loops) interact with each other, although the two strands cannot wrap around each other completely because of the adjacent doublestranded regions (stems). In this study, we exploited DNA kissing-loop interaction for nanotechnological application. We functionalized the vertices of DNA tetrahedrons with DNA stem-loop sequences. The complementary loop sequence design allowed the hybridization of different tetrahedrons via kissingloop interaction, which might be further exploited for nanotechnology applications like cargo transport and logical elements. Importantly, we were able to manipulate the stability of those kissing-loop complexes based on the choice and concentration of cations, the temperature and the number of complementary loops per tetrahedron either at the same or at different vertices. Moreover, variations in loop sequences allowed the characterization of necessary sequences within the loop as well as additional stability control of the kissing compl ... mehr

Zugehörige Institution(en) am KIT Botanisches Institut und Botanischer Garten (BOTANIK)
Publikationstyp Zeitschriftenaufsatz
Jahr 2016
Sprache Englisch
Identifikator DOI: 10.1093/nar/gkw014
ISSN: 0301-5610, 0305-1048, 1362-4962
URN: urn:nbn:de:swb:90-642034
KITopen ID: 1000064203
Erschienen in Nucleic acids research
Band 44
Heft 4
Seiten 1502-1513
Lizenz CC BY-NC 4.0: Creative Commons Namensnennung – Nicht kommerziell 4.0 International
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