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Revolutionizing plant biology: Multiple ways of genome engineering by CRISPR/Cas

Schiml, Simon; Puchta, Holger

Abstract:
The precise manipulation of plant genomes relies on the induction of DNA double-strand breaks by site-specific nucleases to initiate DNA repair reactions that are either based on non-homologous end joining (NHEJ) or homologous recombination (HR). Recently, the CRISPR/Cas system emerged as the most important tool for genome engineering due to its simple structure and its applicability to a wide range of organisms. Here, we review the current status of its various applications in plants, where it is used for the successful generation of stable mutations in a steadily growing number of species through NHEJ. Furthermore, tremendous progress in plant genome engineering by HR was obtained by the setup of replicon mediated and in planta gene targeting techniques. Finally, other complex approaches that rely on the induction of more than one DNA lesion at a time such as paired nickases to avoid off-site effects or controlled genomic deletions are beginning to be applied routinely.


Zugehörige Institution(en) am KIT Botanisches Institut und Botanischer Garten (BOTANIK)
Publikationstyp Zeitschriftenaufsatz
Jahr 2016
Sprache Englisch
Identifikator DOI: 10.1186/s13007-016-0103-0
ISSN: 1746-4811
URN: urn:nbn:de:swb:90-645612
KITopen ID: 1000064561
Erschienen in Plant methods
Band 12
Heft 1
Seiten Art.Nr.: 8
Lizenz CC BY 4.0: Creative Commons Namensnennung 4.0 International
Schlagworte Gene technology, Double-strand break repair, Synthetic nucleases, Targeted mutagenesis, Gene targeting
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