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RNASeq Based Transcriptional Profiling of Pseudomonas aeruginosa PA14 after Short- and Long-Term Anoxic Cultivation in Synthetic Cystic Fibrosis Sputum Medium

Tata, Muralidhar; Wolfinger, Michael T.; Amman, Fabian; Roschanski, Nicole; Dötsch, Andreas; Sonnleitner, Elisabeth; Häussler, Susanne; Bläsi, Udo

Abstract:
The opportunistic human pathogen Pseudomonas aeruginosa can thrive under microaerophilic to anaerobic conditions in the lungs of cystic fibrosis patients. RNA$_{Seq}$ based comparative RNA profiling of the clinical isolate PA14 cultured in synthetic cystic fibrosis medium was performed after planktonic growth (OD$_{600}$ = 2.0; P), 30 min after shift to anaerobiosis (A-30) and after anaerobic biofilm growth for 96h (B-96) with the aim to reveal differentially regulated functions impacting on sustained anoxic biofilm formation as well as on tolerance towards different antibiotics. Most notably, functions involved in sulfur metabolism were found to be up-regulated in B-96 cells when compared to A-30 cells. Based on the transcriptome studies a set of transposon mutants were screened, which revealed novel functions involved in anoxic biofilm growth.In addition, these studies revealed a decreased and an increased abundance of the oprD and the mexCD-oprJ operon transcripts, respectively, in B-96 cells, which may explain their increased tolerance towards meropenem and to antibiotics that are expelled by the MexCD-OprD efflux pump. The OprI ... mehr


Zugehörige Institution(en) am KIT Institut für Funktionelle Grenzflächen (IFG)
Publikationstyp Zeitschriftenaufsatz
Jahr 2016
Sprache Englisch
Identifikator DOI: 10.1371/journal.pone.0147811
ISSN: 1932-6203
URN: urn:nbn:de:swb:90-645965
KITopen ID: 1000064596
HGF-Programm 47.02.06; LK 01
Erschienen in PLoS one
Band 11
Heft 1
Seiten e0147811
Lizenz CC BY 4.0: Creative Commons Namensnennung 4.0 International
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