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Enzyme-Mediated Quenching of the Pseudomonas Quinolone Signal (PQS) Promotes Biofilm Formation of Pseudomonas aeruginosa by Increasing Iron Availability

Tettmann, Beatrix 1; Niewerth, Christine; Kirschhöfer, Frank 1; Neidig, Anke 1; Dötsch, Andreas 1; Brenner-Weiss, Gerald 1; Fetzner, Susanne; Overhage, Joerg 1
1 Institut für Funktionelle Grenzflächen (IFG), Karlsruher Institut für Technologie (KIT)


The 2-alkyl-3-hydroxy-4(1H)-quinolone 2,4-dioxygenase HodC was previously described to cleave the Pseudomonas quinolone signal, PQS, which is exclusively used in the complex quorum sensing (QS) system of Pseudomonas aeruginosa, an opportunistic pathogen employing QS to regulate virulence and biofilm development. Degradation of PQS by exogenous addition of HodC to planktonic cells of P.aeruginosa attenuated production of virulence factors, and reduced virulence inplanta. However, proteolytic cleavager educed the efficacy of HodC. Here, we identified the secreted protease LasB of P.aeruginosa to be responsible for HodC degradation. In static biofilms of the P.aeruginosa PA14 lasB::Tn mutant, the catalytic activity of HodC led to an increase in viable biomass in newly formed but also in established biofilms, and reduced the expression of genes involved in iron metabolism and siderophore production, such as pvdS, pvdL, pvdA, and pvdQ. This is likely due to an increase in the levels of bioavailable iron by degradation of PQS, which is able to sequester iron from the surrounding environment. Thus, HodC, despite ist ability to quench the production of virulence factors, is contraindicated for combating P.aeruginosa biofilms.

Volltext §
DOI: 10.5445/IR/1000065467
DOI: 10.3389/fmicb.2016.01978
Zitationen: 19
Zitationen: 22
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Funktionelle Grenzflächen (IFG)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2016
Sprache Englisch
Identifikator ISSN: 1664-302X
KITopen-ID: 1000065467
HGF-Programm 47.02.06 (POF III, LK 01) Zellpopul.auf Biofunk.Oberflächen IFG
Erschienen in Frontiers in microbiology
Verlag Frontiers Media SA
Band 7
Seiten Art. Nr.: 1978
Bemerkung zur Veröffentlichung Gefördert durch den KIT-Publikationsfonds
Vorab online veröffentlicht am 09.12.2016
Schlagwörter Pseudomonas aeruginosa, Pseudomonas quinolone signal, quorum sensing, quorum quenching, biofilm, PQS dioxygenase
Nachgewiesen in Web of Science
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