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Enzyme-Mediated Quenching of the Pseudomonas Quinolone Signal (PQS) Promotes Biofilm Formation of Pseudomonas aeruginosa by Increasing Iron Availability

Tettmann, Beatrix; Niewerth, Christine; Kirschhöfer, Frank; Neidig, Anke; Dötsch, Andreas; Brenner-Weiss, Gerald; Fetzner, Susanne; Overhage, Joerg

Abstract:
The 2-alkyl-3-hydroxy-4(1H)-quinolone 2,4-dioxygenase HodC was previously described to cleave the Pseudomonas quinolone signal, PQS, which is exclusively used in the complex quorum sensing (QS) system of Pseudomonas aeruginosa, an opportunistic pathogen employing QS to regulate virulence and biofilm development. Degradation of PQS by exogenous addition of HodC to planktonic cells of P.aeruginosa attenuated production of virulence factors, and reduced virulence inplanta. However, proteolytic cleavager educed the efficacy of HodC. Here, we identified the secreted protease LasB of P.aeruginosa to be responsible for HodC degradation. In static biofilms of the P.aeruginosa PA14 lasB::Tn mutant, the catalytic activity of HodC led to an increase in viable biomass in newly formed but also in established biofilms, and reduced the expression of genes involved in iron metabolism and siderophore production, such as pvdS, pvdL, pvdA, and pvdQ. This is likely due to an increase in the levels of bioavailable iron by degradation of PQS, which is able to sequester iron from the surrounding environment. Thus, HodC, despite ist ability to quench the p ... mehr


Zugehörige Institution(en) am KIT Institut für Funktionelle Grenzflächen (IFG)
Publikationstyp Zeitschriftenaufsatz
Jahr 2016
Sprache Englisch
Identifikator DOI: 10.3389/fmicb.2016.01978
ISSN: 1664-302X
URN: urn:nbn:de:swb:90-654670
KITopen ID: 1000065467
HGF-Programm 47.02.06; LK 01
Erschienen in Frontiers in microbiology
Band 7
Seiten Art. Nr.: 1978
Lizenz CC BY 4.0: Creative Commons Namensnennung 4.0 International
Bemerkung zur Veröffentlichung Gefördert durch den KIT-Publikationsfonds
Schlagworte Pseudomonas aeruginosa, Pseudomonas quinolone signal, quorum sensing, quorum quenching, biofilm, PQS dioxygenase
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