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Amorphous Silica Particles Relevant in Food Industry Influence Cellular Growth and Associated Signaling Pathways in Human Gastric Carcinoma Cells

Wittig, Anja; Gehrke, Helge; Favero, Giorgia Del; Fritz, Eva-Maria; Al-Rawi, Marco 1; Diabaté, Silvia 1; Weiss, Carsten 1; Sami, Haider; Ogris, Manfred; Marko, Doris
1 Institut für Toxikologie und Genetik (ITG), Karlsruher Institut für Technologie (KIT)

Abstract:

Nanostructured silica particles are commonly used in biomedical and biotechnical fields, as well as, in cosmetics and food industry. Thus, their environmental and health impacts are of great interest and effects after oral uptake are only rarely investigated. In the present study, the toxicological effects of commercially available nano-scaled silica with a nominal primary diameter of 12 nm were investigated on the human gastric carcinoma cell line GXF251L. Besides the analysis of cytotoxic and proliferative effects and the comparison with effects of particles with a nominal primary diameter of 200 nm, emphasis was also given to their influence on the cellular epidermal growth factor receptor (EGFR) and mitogen-activated protein kinases (MAPK) signaling pathways—both of them deeply involved in the regulation of cellular processes like cell cycle progression, differentiation or proliferation. The investigated silica nanoparticles (NPs) were found to stimulate cell proliferation as measured by microscopy and the sulforhodamine B assay. In accordance, the nuclear level of the proliferation marker Ki-67 was enhanced in a concentration-dependent manner. ... mehr


Verlagsausgabe §
DOI: 10.5445/IR/1000066754
Veröffentlicht am 16.03.2018
Originalveröffentlichung
DOI: 10.3390/nano7010018
Scopus
Zitationen: 15
Web of Science
Zitationen: 14
Dimensions
Zitationen: 14
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Toxikologie und Genetik (ITG)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2017
Sprache Englisch
Identifikator ISSN: 2079-4991
urn:nbn:de:swb:90-667541
KITopen-ID: 1000066754
HGF-Programm 47.01.01 (POF III, LK 01) Biol.Netzwerke u.Synth.Regulat. ITG+ITC
Erschienen in Nanomaterials
Verlag MDPI
Band 7
Heft 1
Seiten Art. Nr.: 18
Vorab online veröffentlicht am 13.01.2017
Nachgewiesen in Web of Science
Scopus
Dimensions
Globale Ziele für nachhaltige Entwicklung Ziel 3 – Gesundheit und Wohlergehen
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