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Functional analysis of Protocadherin18a in zebrafish early development

Bösze, Bernadett

Abstract: “It is not birth, marriage, or death, but gastrulation, which is truly the most important time in your life.” (Lewis Wolpert, 1986). Gastrulation is a pivotal phase of early embryonic development and leads to the formation of the defining axial structure of all Chordates: the notochord. It is essentially the result of major cell rearrangements and is driven by the coordinated movement of mesodermal cells, often referred to as collective cell migration. Cohesion is guided by various factors and stays connected via cadherin-based junctions. In recent years, significant interest has been focused on the largest group within the cadherins superfamily, the protocadherins. The presence of protocadherins in the nervous system has commanded the most attention while little is known about their role in cell-cell adhesions in early development. In this work, I defined the role of Pcdh18a, a novel member of protocadherins in zebrafish gastrulation. Pcdh18a is expressed in a confined cell group – termed the notochord tip cells and exhibit strong connection to the trailing notochord progenitors. At the molecular level, Pcdh18a mediates the recycling of E-cadherin and thereby affects the migratory capabilities of cells. Using the Cellular Potts model, we were able to simulate the migration of mesodermal cells in early zebrafish development. Our model predicted that high motility and strong adhesiveness of Pcdh18a-positive prechordal plate is a prerequisite of proper axis formation during gastrulation.

Zugehörige Institution(en) am KIT Institut für Toxikologie und Genetik (ITG)
Publikationstyp Hochschulschrift
Jahr 2016
Sprache Englisch
Identifikator DOI(KIT): 10.5445/IR/1000067480
URN: urn:nbn:de:swb:90-674806
KITopen ID: 1000067480
Verlag Karlsruhe
Umfang XIII, 85 S.
Abschlussart Dissertation
Fakultät Fakultät für Chemie und Biowissenschaften (CHEM-BIO)
Institut Institut für Toxikologie und Genetik (ITG)
Referent/Betreuer Dr. S. Scholpp
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