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Protein-based fluorescent nanoparticles for super-resolution STED imaging of live cells

Shang, L. 1,2; Gao, P. 1,2; Wang, H. 1; Popescu, R. 3; Gerthsen, D. 3; Nienhaus, G. U. ORCID iD icon 1,2
1 Institut für Angewandte Physik (APH), Karlsruher Institut für Technologie (KIT)
2 Institut für Nanotechnologie (INT), Karlsruher Institut für Technologie (KIT)
3 Laboratorium für Elektronenmikroskopie (LEM), Karlsruher Institut für Technologie (KIT)

Abstract:

Development of nanoparticles for super-resolution imaging (sriNPs) can greatly enrich the toolbox of robust optical probes for biological studies. Moreover, sriNPs enable us to monitor the behavior of engineered nanomaterials in complex biological environments with high spatial resolution, which is important for advancing our understanding of nano–bio interactions. Up to now, reports on sriNPs have been scarce. In this work, we report a facile strategy to prepare protein-based fluorescent NPs that can be utilized as probes in super-resolution microscopy. The method is simple and straightforward, and easily extendible to other types of fluorophores. By using Atto647N–transferrin NPs as an example, we have achieved a roughly four-fold resolution improvement by using STED nanoscopy. These protein-based sriNPs possess excellent biocompatibility, good colloidal stability and photostability, making them attractive candidates for biological studies. Moreover, STED nanoscopy enables the precise imaging of NP structures in living cells, and revealed the co-existence of multiple NPs within one endosomal vesicle.


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Originalveröffentlichung
DOI: 10.1039/c6sc04664a
Scopus
Zitationen: 36
Web of Science
Zitationen: 33
Dimensions
Zitationen: 36
Zugehörige Institution(en) am KIT Institut für Angewandte Physik (APH)
Institut für Nanotechnologie (INT)
Institut für Toxikologie und Genetik (ITG)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2017
Sprache Englisch
Identifikator ISSN: 2041-6520, 2041-6539
KITopen-ID: 1000067560
HGF-Programm 43.23.01 (POF III, LK 01) Advanced Optical Lithography+Microscopy
Erschienen in Chemical science
Verlag Royal Society of Chemistry (RSC)
Band 8
Heft 3
Seiten 2396-2400
Nachgewiesen in Scopus
Dimensions
Web of Science
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