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Phospholipid arrays on porous polymer coatings generated by micro-contact spotting

Sekula-Neuner, Sylwia; de Freitas, Monica; Tröster, Lea-Marie; Jochum, Tobias; Levkin, Pavel A; Hirtz, Michael; Fuchs, Harald

Abstract:
Nanoporous poly(2-hydroxyethyl methacrylate-co-ethylene dimethacrylate) (HEMA-EDMA) is used as a 3D mesh for spotting lipid arrays. Its porous structure is an ideal matrix for lipid ink to infiltrate, resulting in higher fluorescent signal intensity as compared to similar arrays on strictly 2D substrates like glass. The embedded lipid arrays show high stability against washing steps, while still being accessible for protein and antibody binding. To characterize binding to polymer-embedded lipids we have applied Streptavidin as well as biologically important biotinylated androgen receptor binding onto 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine-N-(cap biotinyl) (Biotinyl Cap PE) and anti-DNP IgE recognition of 2,4-dinitrophenyl[1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine-N-[6-[(2,4-dinitrophenyl)amino]hexanoyl] (DNP)] antigen. This approach adds lipid arrays to the range of HEMA polymer applications and makes this solid substrate a very attractive platform for a variety of bio-applications.


Zugehörige Institution(en) am KIT Institut für Nanotechnologie (INT)
Institut für Organische Chemie (IOC)
Institut für Toxikologie und Genetik (ITG)
Karlsruhe Nano Micro Facility (KNMF)
Laboratorium für Applikationen der Synchrotronstrahlung (LAS)
Institut für Photonenforschung und Synchrotronstrahlung (IPS)
Publikationstyp Zeitschriftenaufsatz
Jahr 2017
Sprache Englisch
Identifikator ISSN: 2190-4286
KITopen ID: 1000068515
HGF-Programm 43.22.03; LK 01
Erschienen in Beilstein journal of nanotechnology
Band 8
Seiten 715–722
Schlagworte microcontact cantilever spotting, phospholipids, polymeric porous support, polymethacrylate
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