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Phospholipid arrays on porous polymer coatings generated by micro-contact spotting

Sekula-Neuner, Sylwia; de Freitas, Monica; Tröster, Lea-Marie; Jochum, Tobias; Levkin, Pavel A; Hirtz, Michael; Fuchs, Harald

Abstract:
Nanoporous poly(2-hydroxyethyl methacrylate-co-ethylene dimethacrylate) (HEMA-EDMA) is used as a 3D mesh for spotting lipid arrays. Its porous structure is an ideal matrix for lipid ink to infiltrate, resulting in higher fluorescent signal intensity as compared to similar arrays on strictly 2D substrates like glass. The embedded lipid arrays show high stability against washing steps, while still being accessible for protein and antibody binding. To characterize binding to polymer-embedded lipids we have applied Streptavidin as well as biologically important biotinylated androgen receptor binding onto 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine-N-(cap biotinyl) (Biotinyl Cap PE) and anti-DNP IgE recognition of 2,4-dinitrophenyl[1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine-N-[6-[(2,4-dinitrophenyl)amino]hexanoyl] (DNP)] antigen. This approach adds lipid arrays to the range of HEMA polymer applications and makes this solid substrate a very attractive platform for a variety of bio-applications.

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Verlagsausgabe §
DOI: 10.5445/IR/1000068515
Veröffentlicht am 16.03.2018
Originalveröffentlichung
DOI: 10.3762/bjnano.8.75
Scopus
Zitationen: 4
Web of Science
Zitationen: 4
Coverbild
Zugehörige Institution(en) am KIT Institut für Organische Chemie (IOC)
Laboratorium für Applikationen der Synchrotronstrahlung (LAS)
Institut für Toxikologie und Genetik (ITG)
Institut für Nanotechnologie (INT)
Institut für Photonenforschung und Synchrotronstrahlung (IPS)
Karlsruhe Nano Micro Facility (KNMF)
Publikationstyp Zeitschriftenaufsatz
Jahr 2017
Sprache Englisch
Identifikator ISSN: 2190-4286
urn:nbn:de:swb:90-685159
KITopen-ID: 1000068515
HGF-Programm 43.22.03 (POF III, LK 01)
Erschienen in Beilstein journal of nanotechnology
Band 8
Seiten 715–722
Projektinformation DropCellArray (EU, FP7, 337077)
Schlagworte microcontact cantilever spotting, phospholipids, polymeric porous support, polymethacrylate
Nachgewiesen in Web of Science
Scopus
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