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Enhanced cellular uptake of size-separated lipophilic silicon nanoparticles

Kusi-Appiah, A. E.; Mastronardi, M. L.; Qian, C.; Chen, K. K.; Ghazanfari, L.; Prommapan, P.; Kübel, C.; Ozin, G. A.; Lenhert, S.

Specific size, shape and surface chemistry influence the biological activity of nanoparticles. In the case of lipophilic nanoparticles, which are widely used in consumer products, there is evidence that particle size and formulation influences skin permeability and that lipophilic particles smaller than 6 nm can embed in lipid bilayers. Since most nanoparticle synthetic procedures result in mixtures of different particles, post-synthetic purification promises to provide insights into nanostructure-function relationships. Here we used size-selective precipitation to separate lipophilic allyl-benzyl-capped silicon nanoparticles into monodisperse fractions within the range of 1 nm to 5 nm. We measured liposomal encapsulation and cellular uptake of the monodisperse particles and found them to have generally low cytotoxicities in Hela cells. However, specific fractions showed reproducibly higher cytotoxicity than other fractions as well as the unseparated ensemble. Measurements indicate that the cytotoxicity mechanism involves oxidative stress and the differential cytotoxicity is due to enhanced cellular uptake by specific fractions. The results indicate that specific particles, with enhanced suitability for incorporation into lipophilic regions of liposomes and subsequent in vitro delivery to cells, are enriched in certain fractions.

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Verlagsausgabe §
DOI: 10.5445/IR/1000068731
Veröffentlicht am 09.03.2018
DOI: 10.1038/srep43731
Zitationen: 3
Web of Science
Zitationen: 3
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Nanotechnologie (INT)
Karlsruhe Nano Micro Facility (KNMF)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2017
Sprache Englisch
Identifikator ISSN: 2045-2322
KITopen-ID: 1000068731
HGF-Programm 49.02.01 (POF III, LK 02)
INT-KNMF Characterisation Lab
Erschienen in Scientific reports
Band 7
Seiten 43731
Nachgewiesen in Web of Science
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