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Electrode-assisted acetoin production in a metabolically engineered Escherichia coli strain

Förster, A. H.; Beblawy, S.; Golitsch, F.; Gescher, J.

Abstract:
Background
This paper describes the metabolic engineering of Escherichia coli for the anaerobic fermentation of glucose to acetoin. Acetoin has well-established applications in industrial food production and was suggested to be a platform chemical for a bio-based economy. However, the biotechnological production is often hampered by the simultaneous formation of several end products in the absence of an electron acceptor. Moreover, typical production strains are often potentially pathogenic. The goal of this study was to overcome these limitations by establishing an electrode-assisted fermentation process in E. coli. Here, the surplus of electrons released in the production process is transferred to an electrode as anoxic and non-depletable electron acceptor.

Results
In a first step, the central metabolism was steered towards the production of pyruvate from glucose by deletion of genes encoding for enzymes of central reactions of the anaerobic carbon metabolism (ΔfrdA-D ΔadhE ΔldhA Δpta–ack). Thereafter, the genes for the acetolactate synthase (alsS) and the acetolactate decarboxylase (alsD) were expressed in this strain from a ... mehr


Zugehörige Institution(en) am KIT Institut für Funktionelle Grenzflächen (IFG)
Institut für Angewandte Biowissenschaften (IAB)
Publikationstyp Zeitschriftenaufsatz
Jahr 2017
Sprache Englisch
Identifikator DOI: 10.1186/s13068-017-0745-9
ISSN: 1754-6834
KITopen ID: 1000069023
Erschienen in Biotechnology for biofuels
Band 10
Heft 1
Seiten 65
Schlagworte Electrode-assisted fermentation, Escherichia coli, Bulk chemicals, Acetoin, Metabolic engineering
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