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Increased zinc accumulation in mineralized osteosarcoma tissue measured by confocal synchrotron radiation micro X-ray fluorescence analysis

Rauwolf, Mirjam; Pemmer, Bernhard; Roschger, Andreas; Turyanskaya, Anna; Smolek, Stephan; Maderitsch, Angelika; Hischenhuber, Peter; Foelser, Martin; Simon, Rolf; Lang, Susanna; Puchner, Stephan E.; Windhager, Reinhard; Klaushofer, Klaus; Wobrauschek, Peter; Hofstaetter, Jochen G.; Roschger, Paul; Streli, Christina

Abstract:
Abnormal tissue levels of certain trace elements such as zinc (Zn) were reported in various types of cancer. Little is known about the role of Zn in osteosarcoma. Using confocal synchrotron radiation micro X-ray fluorescence analysis, we characterized the spatial distribution of Zn in high-grade sclerosing osteosarcoma of nine patients (four women/five men; seven knee/one humerus/one femur) following chemotherapy and wide surgical resection. Levels were compared with adjacent normal tissue. Quantitative backscattered electron imaging as well as histological examinations was also performed. On average, the ratio of medians of Zn count rates (normalized to calcium) in mineralized tumor tissue was about six times higher than in normal tissue. There was no difference in Zn levels between tumor fraction areas with a low fraction and a high fraction of mineralized tissue, which were clearly depicted using quantitative backscattered electron imaging. Moreover, we found no correlation between the Zn values and the type of tumor regression according to the Salzer-Kuntschik grading. The underlying mechanism of Zn accumulation remains unclear. ... mehr


Zugehörige Institution(en) am KIT ANKA - die Synchrotronstrahlungsquelle am KIT (ANKA)
Institut für Photonenforschung und Synchrotronstrahlung (IPS)
Publikationstyp Zeitschriftenaufsatz
Jahr 2017
Sprache Englisch
Identifikator DOI: 10.1002/xrs.2727
ISSN: 0049-8246, 1097-4539
KITopen ID: 1000069362
HGF-Programm 56.03.30; LK 01
Erschienen in X-ray spectrometry
Band 46
Heft 1
Seiten 56–62
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