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Screening and Optimizing Antimicrobial Peptides by Using SPOT-Synthesis

López-Pérez, Paula M.; Grimsey, Elizabeth; Bourne, Luc; Mikut, Ralf ORCID iD icon 1; Hilpert, Kai
1 Institut für Angewandte Informatik (IAI), Karlsruher Institut für Technologie (KIT)


Peptide arrays on cellulose are a powerful tool to investigate peptide interactions with a number of different molecules, for examples antibodies, receptors or enzymes. Such peptide arrays can also be used to study interactions with whole cells. In this review, we focus on the interaction of small antimicrobial peptides with bacteria. Antimicrobial peptides (AMPs) can kill multidrug-resistant (MDR) human pathogenic bacteria and therefore could be next generation antibiotics targeting MDR bacteria. We describe the screen and the result of different optimization strategies of peptides cleaved from the membrane. In addition, screening of antibacterial activity of peptides that are tethered to the surface is discussed. Surface-active peptides can be used to protect surfaces from bacterial infections, for example implants.

Volltext §
DOI: 10.5445/IR/1000069478
DOI: 10.3389/fchem.2017.00025
Zitationen: 31
Web of Science
Zitationen: 30
Zitationen: 35
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Angewandte Informatik (IAI)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2017
Sprache Englisch
Identifikator ISSN: 2296-2646
KITopen-ID: 1000069478
HGF-Programm 47.01.02 (POF III, LK 01) Biol.Netzwerke u.Synth.Regulat. IAI
Erschienen in Frontiers in Chemistry
Verlag Frontiers Media SA
Band 5
Seiten Article 25
Bemerkung zur Veröffentlichung Gefördert durch den KIT-Publikationsfonds
Vorab online veröffentlicht am 12.04.2017
Schlagwörter SPOT-synthesis, antimicrobial peptides, peptide synthesis, antimicrobial screening, peptide libraries, substitution analysis, multi-drug resistance, tethered peptides
Nachgewiesen in Dimensions
Web of Science
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