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Screening and Optimizing Antimicrobial Peptides by Using SPOT-Synthesis

López-Pérez, Paula M.; Grimsey, Elizabeth; Bourne, Luc; Mikut, Ralf; Hilpert, Kai

Abstract:
Peptide arrays on cellulose are a powerful tool to investigate peptide interactions with a number of different molecules, for examples antibodies, receptors or enzymes. Such peptide arrays can also be used to study interactions with whole cells. In this review, we focus on the interaction of small antimicrobial peptides with bacteria. Antimicrobial peptides (AMPs) can kill multidrug-resistant (MDR) human pathogenic bacteria and therefore could be next generation antibiotics targeting MDR bacteria. We describe the screen and the result of different optimization strategies of peptides cleaved from the membrane. In addition, screening of antibacterial activity of peptides that are tethered to the surface is discussed. Surface-active peptides can be used to protect surfaces from bacterial infections, for example implants.


Zugehörige Institution(en) am KIT Institut für Angewandte Informatik (IAI)
Publikationstyp Zeitschriftenaufsatz
Jahr 2017
Sprache Englisch
Identifikator DOI: 10.3389/fchem.2017.00025
ISSN: 2296-2646
URN: urn:nbn:de:swb:90-694781
KITopen ID: 1000069478
HGF-Programm 47.01.02; LK 01
Erschienen in Frontiers in Chemistry
Band 5
Seiten Article 25
Lizenz CC BY 4.0: Creative Commons Namensnennung 4.0 International
Bemerkung zur Veröffentlichung Gefördert durch den KIT-Publikationsfonds
Schlagworte SPOT-synthesis, antimicrobial peptides, peptide synthesis, antimicrobial screening, peptide libraries, substitution analysis, multi-drug resistance, tethered peptides
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