KIT | KIT-Bibliothek | Impressum | Datenschutz
Open Access Logo
DOI: 10.5445/IR/1000071261

Novel tools for simultaneous optogenetic manipulation and calcium imaging in the zebrafish nervous system

Gheisari, Ali

Abstract (englisch):
The large number of neurons and neural interconnection makes the nervous system a highly dense and complex network. Understanding the functionality of such a network requires not only high-throughput recording of neural activities with cellular resolution but also a non-invasive and precisely defined interaction with the neurons. This work demonstrates two pivotal steps towards these aims:
(i) Developing a fluorescence microscope based on Bessel light-sheet illumination to record neural activities by means of calcium imaging. Chapter II describes the successful development and construction of an in vivo light-sheet fluorescence microscope (in vivo LSFM) based on conventional digitally laser scanning light-sheet microscopy (DSLM) with interchangeable Gaussian and Bessel illumination modalities to compare the performance of both methods. The Bessel illumination modality in comparison to the Gaussian one reveals not only a two-fold improvement in axial resolution but also a reduction by the factor of 4 in the shadowing artifact and consequently, 35 times improvement in detecting the correct neural activities from the calcium signals.
... mehr

Zugehörige Institution(en) am KIT Zoologisches Institut (ZOO)
Publikationstyp Hochschulschrift
Jahr 2017
Sprache Englisch
Identifikator URN: urn:nbn:de:swb:90-712618
KITopen-ID: 1000071261
Verlag Karlsruhe
Umfang 151 S.
Abschlussart Dissertation
Fakultät Fakultät für Chemie und Biowissenschaften (CHEM-BIO)
Institut Zoologisches Institut (ZOO)
Prüfungsdatum 27.04.2017
Referent/Betreuer Prof. M. Bastmeyer (KIT); Prof. F. S. Pavone (Univ. d. Studi, Firenze)
Bemerkung zur Veröffentlichung Zugl.: Florenz, Univ. d. Studi, Diss.
Schlagworte Fluorescence, Light Microscopy, Optical sectioning, Light sheet microscopy, Bessel Light sheet, Neuronal activity recording, in vivo imaging, Optogenetics, transgenic zebrafish, Chlamydomonas channelrhodopsin1, Volvox channelrhodopsin1, C1V1
KIT – Die Forschungsuniversität in der Helmholtz-Gemeinschaft KITopen Landing Page