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URN: urn:nbn:de:swb:90-713042
Originalveröffentlichung
DOI: 10.3389/fmicb.2017.01282
Scopus
Zitationen: 16
Web of Science
Zitationen: 14

Occurrence of the mcr-1 Colistin Resistance Gene and other Clinically Relevant Antibiotic Resistance Genes in Microbial Populations at Different Municipal Wastewater Treatment Plants in Germany

Hembach, Norman; Schmid, Ferdinand; Alexander, Johannes; Hiller, Christian; Rogall, Eike T.; Schwartz, Thomas

Abstract:
Seven wastewater treatment plants (WWTPs) with different population equivalents and catchment areas were screened for the prevalence of the colistin resistance gene mcr-1 mediating resistance against last resort antibiotic polymyxin E. The abundance of the plasmid-associated mcr-1 gene in total microbial populations during water treatment processes was quantitatively analyzed by qPCR analyses. The presence of the colistin resistance gene was documented for all of the influent wastewater samples of the seven WWTPs. In some cases the mcr-1 resistance gene was also detected in effluent samples of the WWTPs after conventional treatment reaching the aquatic environment. In addition to the occurrence of mcr-1 gene, CTX-M-32, blaTEM, CTX-M, tetM, CMY-2, and ermB genes coding for clinically relevant antibiotic resistances were quantified in higher abundances in all WWTPs effluents. In parallel, the abundances of Acinetobacter baumannii, Klebsiella pneumoniae, and Escherichia coli were quantified via qPCR using specific taxonomic gene markers which were detected in all influent and effluent wastewaters in significant densities. Hence, opport ... mehr


Zugehörige Institution(en) am KIT Institut für Funktionelle Grenzflächen (IFG)
Publikationstyp Zeitschriftenaufsatz
Jahr 2017
Sprache Englisch
Identifikator ISSN: 1664-302X
KITopen ID: 1000071304
HGF-Programm 47.02.06; LK 01
Erschienen in Frontiers in microbiology
Band 8
Seiten Art.Nr. 1282
Bemerkung zur Veröffentlichung Gefördert durch den KIT-Publikationsfonds
Schlagworte Colistin resistance, antibiotic resistance genes, opportunistic bacteria, wastewater treatment, qPCR
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