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Characterisation of a stable laboratory co-culture of acidophilic nanoorganisms

Krause, S.; Bremges, A.; Münch, P. C.; McHardy, A. C.; Gescher, J.

Abstract:
This study describes the laboratory cultivation of ARMAN (Archaeal Richmond Mine Acidophilic
Nanoorganisms). After 2.5 years of successive transfers in an anoxic medium containing ferric sulfate
as an electron acceptor, a consortium was attained that is comprised of two members of the order
Thermoplasmatales, a member of a proposed ARMAN group, as well as a fungus. The 16S rRNA identity
of one archaeon is only 91.6% compared to the most closely related isolate Thermogymnomonas
acidicola. Hence, this organism is the first member of a new genus. The enrichment culture is dominated
by this microorganism and the ARMAN. The third archaeon in the community seems to be present in
minor quantities and has a 100% 16S rRNA identity to the recently isolated Cuniculiplasma divulgatum.
The enriched ARMAN species is most probably incapable of sugar metabolism because the key genes
for sugar catabolism and anabolism could not be identified in the metagenome. Metatranscriptomic
analysis suggests that the TCA cycle funneled with amino acids is the main metabolic pathway used by
the archaea of the community. Microscopic analysis revealed t ... mehr


Zugehörige Institution(en) am KIT Institut für Biologische Grenzflächen (IBG)
Institut für Angewandte Biowissenschaften (IAB)
Publikationstyp Zeitschriftenaufsatz
Jahr 2017
Sprache Englisch
Identifikator DOI: 10.1038/s41598-017-03315-6
ISSN: 2045-2322
URN: urn:nbn:de:swb:90-715047
KITopen ID: 1000071504
Erschienen in Scientific reports
Band 7
Heft 1
Seiten Art. Nr.: 3289
Lizenz CC BY 4.0: Creative Commons Namensnennung 4.0 International
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