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DOI: 10.5445/IR/1000071791

Tracking protein function with sodium multi quantum spectroscopy in a 3D-tissue culture based on microcavity arrays

Neubauer, Andreas; Nies, Cordula; Schepkin, Victor D.; Hu, Ruomin; Malzacher, Matthias; Chacón-Caldera, Jorge; Thiele, David; Gottwald, Eric; Schad, Lothar R.

Abstract:
The aim of this study was to observe the effects of strophanthin induced inhibition of the Na-/KATPase
in liver cells using a magnetic resonance (MR) compatible bioreactor. A microcavity array with
a high density three-dimensional cell culture served as a functional magnetic resonance imaging (MRI)
phantom for sodium multi quantum (MQ) spectroscopy. Direct contrast enhanced (DCE) MRI revealed
the homogenous distribution of biochemical substances inside the bioreactor. NMR experiments using
advanced bioreactors have advantages with respect to having full control over a variety of physiological
parameters such as temperature, gas composition and fluid flow. Simultaneous detection of single
quantum (SQ) and triple quantum (TQ) MR signals improves accuracy and was achieved by application
of a pulse sequence with a time proportional phase increment (TQTPPI). The time course of the Na-/KATPase
inhibition in the cell culture was demonstrated by the corresponding alterations of sodium TQ/
SQ MR signals.


Zugehörige Institution(en) am KIT Institut für Biologische Grenzflächen (IBG)
Institut für Funktionelle Grenzflächen (IFG)
Publikationstyp Zeitschriftenaufsatz
Jahr 2017
Sprache Englisch
Identifikator ISSN: 2045-2322
URN: urn:nbn:de:swb:90-717915
KITopen ID: 1000071791
HGF-Programm 47.02.05; LK 01
Erschienen in Scientific reports
Band 7
Heft 1
Seiten Art. Nr. 3943
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