KIT | KIT-Bibliothek | Impressum | Datenschutz

Tracking protein function with sodium multi quantum spectroscopy in a 3D-tissue culture based on microcavity arrays

Neubauer, Andreas; Nies, Cordula 1; Schepkin, Victor D.; Hu, Ruomin; Malzacher, Matthias; Chacón-Caldera, Jorge; Thiele, David 2; Gottwald, Eric ORCID iD icon 1; Schad, Lothar R.
1 Institut für Funktionelle Grenzflächen (IFG), Karlsruher Institut für Technologie (KIT)
2 Institut für Biologische Grenzflächen (IBG), Karlsruher Institut für Technologie (KIT)


The aim of this study was to observe the effects of strophanthin induced inhibition of the Na-/KATPase
in liver cells using a magnetic resonance (MR) compatible bioreactor. A microcavity array with
a high density three-dimensional cell culture served as a functional magnetic resonance imaging (MRI)
phantom for sodium multi quantum (MQ) spectroscopy. Direct contrast enhanced (DCE) MRI revealed
the homogenous distribution of biochemical substances inside the bioreactor. NMR experiments using
advanced bioreactors have advantages with respect to having full control over a variety of physiological
parameters such as temperature, gas composition and fluid flow. Simultaneous detection of single
quantum (SQ) and triple quantum (TQ) MR signals improves accuracy and was achieved by application
of a pulse sequence with a time proportional phase increment (TQTPPI). The time course of the Na-/KATPase
inhibition in the cell culture was demonstrated by the corresponding alterations of sodium TQ/
SQ MR signals.

Volltext §
DOI: 10.5445/IR/1000071791
DOI: 10.1038/s41598-017-04226-2
Zitationen: 10
Web of Science
Zitationen: 9
Zitationen: 10
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Biologische Grenzflächen (IBG)
Institut für Funktionelle Grenzflächen (IFG)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2017
Sprache Englisch
Identifikator ISSN: 2045-2322
KITopen-ID: 1000071791
HGF-Programm 47.02.05 (POF III, LK 01) Zellpopul.auf Biofunk.Oberflächen IBG-5
Erschienen in Scientific reports
Verlag Nature Research
Band 7
Seiten Art. Nr. 3943
Vorab online veröffentlicht am 21.06.2017
Nachgewiesen in Web of Science
KIT – Die Forschungsuniversität in der Helmholtz-Gemeinschaft
KITopen Landing Page