KIT | KIT-Bibliothek | Impressum | Datenschutz

Single microtubules and small networks become significantly stiffer on short time-scales upon mechanical stimulation

Koch, M. D.; Schneider, N.; Nick, P.; Rohrbach, A.

Abstract:
The transfer of mechanical signals through cells is a complex phenomenon. To uncover a new mechanotransduction pathway, we study the frequency-dependent transport of mechanical stimuli by single microtubules and small networks in a bottom-up approach using optically trapped beads as anchor points. We interconnected microtubules to linear and triangular geometries to perform micro-rheology by defined oscillations of the beads relative to each other. We found a substantial stiffening of single filaments above a characteristic transition frequency of 1–30 Hz depending on the filament’s molecular composition. Below this frequency, filament elasticity only depends on its contour and persistence length. Interestingly, this elastic behavior is transferable to small networks, where we found the surprising effect that linear two filament connections act as transistor-like, angle dependent momentum filters, whereas triangular networks act as stabilizing elements. These observations implicate that cells can tune mechanical signals by temporal and spatial filtering stronger and more flexibly than expected.

Open Access Logo


Volltext §
DOI: 10.5445/IR/1000072169
Originalveröffentlichung
DOI: 10.1038/s41598-017-04415-z
Scopus
Zitationen: 1
Web of Science
Zitationen: 2
Seitenaufrufe: 8
seit 04.05.2018
Downloads: 20
seit 02.09.2017
Zugehörige Institution(en) am KIT Botanisches Institut und Botanischer Garten (BOTANIK)
Publikationstyp Zeitschriftenaufsatz
Jahr 2017
Sprache Englisch
Identifikator ISSN: 2045-2322
urn:nbn:de:swb:90-721696
KITopen-ID: 1000072169
Erschienen in Scientific reports
Band 7
Heft 1
Seiten Art.Nr.: 4229
Nachgewiesen in Scopus
Web of Science
KIT – Die Forschungsuniversität in der Helmholtz-Gemeinschaft
KITopen Landing Page