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The protein corona on nanoparticles as viewed from a nanoparticle-sizing perspective

Wang, Haixia 1; Lin, Youhui 1,2; Nienhaus, Karin 1; Nienhaus, G. Ulrich 1,2,3
1 Institut für Angewandte Physik (APH), Karlsruher Institut für Technologie (KIT)
2 Institut für Nanotechnologie (INT), Karlsruher Institut für Technologie (KIT)
3 Institut für Toxikologie und Genetik (ITG), Karlsruher Institut für Technologie (KIT)

Abstract (englisch):

Most surfaces of engineered nanoparticles (NPs) are reactive toward biomolecules. Therefore, whenever NPs become immersed in biological fluids, proteins and other biomolecules bind to the NP surface, forming an adsorption layer (biomolecular corona) that modifies the NPs’ physicochemical properties and subsequent interactions with living systems. Its exploration is a formidable endeavor owing to the enormous diversity of engineered NPs in terms of their physicochemical properties and the vast number of biomolecules available in biofluids that may bind to NPs with widely different strengths. Significant progress has been made in our understanding of the biomolecular corona, but even very basic issues are still controversially debated. In fact, there are divergent views of its microscopic structure and dynamics, even on physical properties, such as its thickness. As an example, there is no agreement on whether proteins form monolayers or multilayers upon adsorption. In our quantitative studies of NP–protein interactions by in situ fluorescence correlation spectroscopy (FCS) with highly defined model NPs and important serum proteins, we have universally observed protein monolayer formation around NPs under saturation or even oversaturation conditions. ... mehr

DOI: 10.1002/wnan.1500
Zitationen: 49
Web of Science
Zitationen: 39
Zitationen: 53
Zugehörige Institution(en) am KIT Institut für Angewandte Physik (APH)
Institut für Nanotechnologie (INT)
Institut für Toxikologie und Genetik (ITG)
Publikationstyp Zeitschriftenaufsatz
Publikationsdatum 26.10.2017
Sprache Englisch
Identifikator ISSN: 1939-0041, 1939-5116
KITopen-ID: 1000076141
HGF-Programm 43.23.01 (POF III, LK 01) Advanced Optical Lithography+Microscopy
Erschienen in Wiley interdisciplinary reviews / Nanomedicine and Nanobiotechnology
Verlag John Wiley and Sons
Seiten Art.Nr.: e1500
Nachgewiesen in Dimensions
Web of Science
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