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The ECHO of Cell Polarity - Revealing intra- and extracellular factors involved in polarity and axis formation de novo in tobacco cells

Brochhausen, Linda Aimee

Abstract (englisch):

Within all prokaryotic kingdoms cell polarity plays an indispensable role for various cellular functions. Failure in cell polarity can often result in serious diseases. Thus, understanding the mechanism behind polarity formation assigns a fundamental task to basic research.
In contrast to mammalian cells, directionality is a fixed characteristic of plant cells. However, after division, this “direction” must be re-established. How cells acquire polarity and axis presents a central question of plant morphogenesis and represents the aim of this study. Therefore, the role of extracellular as well as intracellular candidates for axis formation was analyzed by using an experimental system based on regenerating protoplasts, where the induction of a cell axis de novo can be followed by quantification of specific regeneration stages.
Nuclear migration and positioning are crucial for the morphogenesis of plant cells. The potential role of nuclear positioning for polarity induction was addressed by using overexpression of fluorescently tagged extranuclear (perinuclear actin basket, KCH kinesins) as well as intranuclear (histone H2B) factors of nuclear positioning. ... mehr

Volltext §
DOI: 10.5445/IR/1000076953
Veröffentlicht am 28.11.2017
Cover der Publikation
Zugehörige Institution(en) am KIT Fakultät für Chemie und Biowissenschaften – Botanisches Institut und Botanischer Garten (BOTANIK)
Publikationstyp Hochschulschrift
Publikationsjahr 2017
Sprache Englisch
Identifikator urn:nbn:de:swb:90-769532
KITopen-ID: 1000076953
Verlag Karlsruher Institut für Technologie (KIT)
Umfang XVII, 122, 1 S.
Art der Arbeit Dissertation
Fakultät Fakultät für Chemie und Biowissenschaften (CHEM-BIO)
Institut Fakultät für Chemie und Biowissenschaften – Botanisches Institut und Botanischer Garten (BOTANIK)
Prüfungsdatum 17.10.2017
Referent/Betreuer Nick, P.
KIT – Die Forschungsuniversität in der Helmholtz-Gemeinschaft
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