KIT | KIT-Bibliothek | Impressum
Open Access Logo
§
Volltext
DOI: 10.5445/IR/1000076953

The ECHO of Cell Polarity - Revealing intra- and extracellular factors involved in polarity and axis formation de novo in tobacco cells

Brochhausen, Linda Aimee

Abstract (englisch):
Within all prokaryotic kingdoms cell polarity plays an indispensable role for various cellular functions. Failure in cell polarity can often result in serious diseases. Thus, understanding the mechanism behind polarity formation assigns a fundamental task to basic research.
In contrast to mammalian cells, directionality is a fixed characteristic of plant cells. However, after division, this “direction” must be re-established. How cells acquire polarity and axis presents a central question of plant morphogenesis and represents the aim of this study. Therefore, the role of extracellular as well as intracellular candidates for axis formation was analyzed by using an experimental system based on regenerating protoplasts, where the induction of a cell axis de novo can be followed by quantification of specific regeneration stages.
Nuclear migration and positioning are crucial for the morphogenesis of plant cells. The potential role of nuclear positioning for polarity induction was addressed by using overexpression of fluorescently tagged extranuclear (perinuclear actin basket, KCH kinesins) as well as intranuclear (histone H2B) factors ... mehr


Zugehörige Institution(en) am KIT Botanisches Institut und Botanischer Garten (BOTANIK)
Publikationstyp Hochschulschrift
Jahr 2017
Sprache Englisch
Identifikator URN: urn:nbn:de:swb:90-769532
KITopen ID: 1000076953
Verlag Karlsruhe
Umfang XVII, 122, 1 S.
Abschlussart Dissertation
Fakultät Fakultät für Chemie und Biowissenschaften (CHEM-BIO)
Institut Botanisches Institut und Botanischer Garten (BOTANIK)
Prüfungsdatum 17.10.2017
Referent/Betreuer Prof. P. Nick
KIT – Die Forschungsuniversität in der Helmholtz-Gemeinschaft KITopen Landing Page