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DOI: 10.5445/IR/1000077874

Immobilization of Cellulase on Magnetic Nanocarriers

Roth, Hans-Christian; Schwaminger, Sebastian P.; Peng, Fei; Berensmeier, Sonja

The constant increase in the number of sustainable products on the global markets demands new biotechnological processing strategies such as the purification and recovery of biocatalysts. Superparamagnetic iron oxide nanoparticles exhibit excellent recovery properties as carrier materials in enzyme catalysis. We present the simple and fast electrostatic assembly of cellulase (CEL) and low-priced silica-coated magnetic nanoparticles, which demonstrates stable enzyme bonding and excellent colloidal stability. The high CEL loading (0.43 g g⁻¹), without leaching of biocatalyst and high recovery yields (75 %), could be sustained over ten magnetic recycling steps. The highlight of this study is the preservation of a high enzymatic activity and, therefore, the outstandingly high lifecycle stability.

Zugehörige Institution(en) am KIT Institut für Bio- und Lebensmitteltechnik (BLT)
Publikationstyp Zeitschriftenaufsatz
Jahr 2016
Sprache Englisch
Identifikator ISSN: 2191-1355, 2191-1363
URN: urn:nbn:de:swb:90-778749
KITopen ID: 1000077874
Erschienen in ChemistryOpen
Band 5
Heft 3
Seiten 183-187
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