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3D Carbon Scaffolds for Neural Stem Cell Culture and Magnetic Resonance Imaging

Fuhrer, Erwin; Bäcker, Anne; Kraft, Stephanie; Gruhl, Friederike J.; Kirsch, Matthias; MacKinnon, Neil; Korvink, Jan G.; Sharma, Swati

Abstract:
3D glassy carbon structures with percolated macropores are obtained by pyrolysis of chemically synthesized cryogels featuring tunable porosity. These batch-fabricated structures are used as scaffolds for culturing neural stem cells (NSCs) and are characterized by magnetic resonance imaging (MRI). With the aid of MRI, the successful cultivation of NSCs on a glassy carbon surface and the precise 3D locations of these cell clusters within the opaque scaffold are demonstrated. MRI also yields pore morphology and porosity analyses, pre- and post-pyrolysis. This integrated approach yields a complete 3D dataset of the NSC network, which enables the visual inspection of the morphological details of individual cell clusters without disturbing them or destroying the scaffold. Reported experimental methodology is expected to have an impact on studies designed to understand the mechanism of neurodegenerative disease (ND) development, and can serve as a protocol for the culture of various other types of cells that display compatibility with glassy carbon surfaces.


Zugehörige Institution(en) am KIT Institut für Mikrostrukturtechnik (IMT)
Publikationstyp Zeitschriftenaufsatz
Jahr 2017
Sprache Englisch
Identifikator ISSN: 2192-2640, 2192-2659
KITopen ID: 1000078265
HGF-Programm 47.01.03; LK 01
Erschienen in Advanced healthcare materials
Band 7
Heft 4
Seiten Art.Nr. 1700915
Vorab online veröffentlicht am 21.12.2017
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