The Karlsruhe Institute of Technology (KIT) and the WEKA AG jointly develop a commercial flowmeter for application in helium cryostats. The flowmeter functions according to a new thermal measurement principle that eliminates all systematic uncertainties and enables self-calibration during real operation. Ideally, the resulting uncertainty of the measured flow rate is only dependent on signal noises, which are typically very small with regard to the measured value. Under real operating conditions, cryoplant-dependent flow rate fluctuations induce an additional uncertainty, which follows from the sensitivity of the method. This paper presents experimental results with helium at temperatures between 30 and 70 K and flow rates in the range of 4 to 12 g/s. The experiments were carried out in a control cryostat of the 2 kW helium refrigerator of the TOSKA test facility at KIT. Inside the cryostat, the new flowmeter was installed in series with a Venturi tube that was used for reference measurements. The measurement results demonstrate the self-calibration capability during real cryoplant operation. The influences of temperature and flow rate fluctuations on the self-calibration uncertainty are discussed.