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DOI: 10.5445/IR/1000080124
DOI: 10.1126/sciadv.1701798

Superresolution and pulse-chase imaging reveal the role of vesicle transport in polar growth of fungal cells

Zhou, Lu; Evangelinos, Minoas; Wernet, Valentin; Eckert, Antonia F.; Ishitsuka, Yuji; Fischer, Reinhard; Nienhaus, G. Ulrich; Takeshita, Norio

Polarized growth of filamentous fungi requires continuous transport of biomolecules to the hyphal tip. To this end, construction materials are packaged in vesicles and transported by motor proteins along microtubules and actin filaments. We have studied these processes with quantitative superresolution localization microscopy of live Aspergillus nidulans cells expressing the photoconvertible protein mEosFPthermo fused to the chitin synthase ChsB. ChsB is mainly located at the Spitzenkörper near the hyphal tip and produces chitin, a key component of the cell wall. We have visualized the pulsatory dynamics of the Spitzenkörper, reflecting vesicle accumulation before exocytosis and their subsequent fusion with the apical plasma membrane. Furthermore, high-speed pulse-chase imaging after photoconversion of mEosFPthermo in a tightly focused spot revealed that ChsB is transported with two different speeds from the cell body to the hyphal tip and vice versa. Comparative analysis using motor protein deletion mutants allowed us to assign the fast movements (7 to 10 μm s−1) to transport of secretory vesicles by kinesin-1, and the slower ones ... mehr

Zugehörige Institution(en) am KIT Institut für Angewandte Physik (APH)
Institut für Nanotechnologie (INT)
Institut für Toxikologie und Genetik (ITG)
Institut für Angewandte Biowissenschaften (IAB)
Publikationstyp Zeitschriftenaufsatz
Jahr 2018
Sprache Englisch
Identifikator ISSN: 2375-2548
URN: urn:nbn:de:swb:90-801242
KITopen ID: 1000080124
HGF-Programm 43.23.01; LK 01
Erschienen in Science advances
Band 4
Heft 1
Seiten e1701798
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