KIT | KIT-Bibliothek | Impressum | Datenschutz

Superresolution and pulse-chase imaging reveal the role of vesicle transport in polar growth of fungal cells

Zhou, Lu 1,2; Evangelinos, Minoas 3; Wernet, Valentin 3; Eckert, Antonia F. 1; Ishitsuka, Yuji 1; Fischer, Reinhard 3; Nienhaus, G. Ulrich 1,2,4; Takeshita, Norio 3
1 Institut für Angewandte Physik (APH), Karlsruher Institut für Technologie (KIT)
2 Institut für Nanotechnologie (INT), Karlsruher Institut für Technologie (KIT)
3 Institut für Angewandte Biowissenschaften (IAB), Karlsruher Institut für Technologie (KIT)
4 Institut für Toxikologie und Genetik (ITG), Karlsruher Institut für Technologie (KIT)


Polarized growth of filamentous fungi requires continuous transport of biomolecules to the hyphal tip. To this end, construction materials are packaged in vesicles and transported by motor proteins along microtubules and actin filaments. We have studied these processes with quantitative superresolution localization microscopy of live Aspergillus nidulans cells expressing the photoconvertible protein mEosFPthermo fused to the chitin synthase ChsB. ChsB is mainly located at the Spitzenkörper near the hyphal tip and produces chitin, a key component of the cell wall. We have visualized the pulsatory dynamics of the Spitzenkörper, reflecting vesicle accumulation before exocytosis and their subsequent fusion with the apical plasma membrane. Furthermore, high-speed pulse-chase imaging after photoconversion of mEosFPthermo in a tightly focused spot revealed that ChsB is transported with two different speeds from the cell body to the hyphal tip and vice versa. Comparative analysis using motor protein deletion mutants allowed us to assign the fast movements (7 to 10 μm s−1) to transport of secretory vesicles by kinesin-1, and the slower ones (2 to 7 μm s−1) to transport by kinesin-3 on early endosomes. ... mehr

Verlagsausgabe §
DOI: 10.5445/IR/1000080124
Veröffentlicht am 12.02.2018
DOI: 10.1126/sciadv.1701798
Zitationen: 31
Web of Science
Zitationen: 31
Zitationen: 39
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Angewandte Biowissenschaften (IAB)
Institut für Angewandte Physik (APH)
Institut für Nanotechnologie (INT)
Institut für Toxikologie und Genetik (ITG)
Universität Karlsruhe (TH) – Interfakultative Einrichtungen (Interfakultative Einrichtungen)
Karlsruhe School of Optics & Photonics (KSOP)
Publikationstyp Zeitschriftenaufsatz
Publikationsdatum 24.01.2018
Sprache Englisch
Identifikator ISSN: 2375-2548
KITopen-ID: 1000080124
HGF-Programm 43.23.01 (POF III, LK 01) Advanced Optical Lithography+Microscopy
Erschienen in Science advances
Verlag American Association for the Advancement of Science (AAAS)
Band 4
Heft 1
Seiten e1701798
Bemerkung zur Veröffentlichung Gefördert durch den KIT-Publikationsfonds
Nachgewiesen in Scopus
Web of Science
KIT – Die Forschungsuniversität in der Helmholtz-Gemeinschaft
KITopen Landing Page