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Cytoplasmic Transport Machinery of the SPF27 Homologue Num1 in Ustilago maydis

Zhou, Lu; Obhof, Theresa; Schneider, Karina; Feldbrügge, Michael; Nienhaus, G. Ulrich; Kämper, Jörg

In the phytopathogenic basidiomycete Ustilago maydis, the Num1 protein has a pivotal function in hyphal morphogenesis. Num1 functions as a core component of the spliceosome-associated Prp19/CDC5 complex (NTC). The interaction of Num1 with the kinesin motor Kin1 suggests a connection between a component of the splicing machinery and cytoplasmic trafficking processes. Previously it was shown that Num1 localizes predominantly in the nucleus; however, due to the diffraction-limited spatial resolution of conventional optical microscopy, it was not possible to attribute the localization to specific structures within the cytoplasm. We have now employed super-resolution localization microscopy to visualize Num1 in the cytoplasm by fusing it to a tandem dimeric Eos fluorescent protein (tdEosFP). The Num1 protein is localized within the cytoplasm with an enhanced density in the vicinity of microtubules. Num1 movement is found predominantly close to the nucleus. Movement is dependent on its interaction partner Kin1, but independent of Kin3. Our results provide strong evidence that, in addition to its involvement in splicing in the nucleus, Num1 has an additional functional role in the cytosol connected to the Kin1 motor protein.

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Verlagsausgabe §
DOI: 10.5445/IR/1000081165
Veröffentlicht am 19.03.2018
DOI: 10.1038/s41598-018-21628-y
Zitationen: 6
Web of Science
Zitationen: 6
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Angewandte Physik (APH)
Institut für Toxikologie und Genetik (ITG)
Institut für Nanotechnologie (INT)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2018
Sprache Englisch
Identifikator ISSN: 2045-2322
KITopen-ID: 1000081165
HGF-Programm 43.23.01 (POF III, LK 01)
Advanced Optical Lithography+Microscopy
Erschienen in Scientific reports
Band 8
Heft 1
Seiten Art.Nr. 3611
Bemerkung zur Veröffentlichung Gefördert durch den KIT-Publikationsfonds
Vorab online veröffentlicht am 26.02.2018
Schlagwörter Fungal biology; Intracellular movement; Optical imaging
Nachgewiesen in Scopus
Web of Science
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