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Emissions from a modern log wood masonry heater and wood pellet boiler : Composition and biological impact on air-liquid interface exposed human lung cancer cells

Kanashova, Tamara; Sippula, Olli; Oeder, Sebastian; Streibel, Thorsten; Passig, Johannes; Czech, Hendryk; Kaoma, Tony; Sapcariu, Sean C.; Dilger, Marco; Paur, Hanns-Rudolf; Schlager, Christoph; Mülhopt, Sonja; Weiss, Carsten; Schmidt-Weber, Carsten; Traidl-Hoffmann, Claudia; Michalke, Bernhard; Krebs, Tobias; Karg, Erwin; Jakobi, Gert; Scholtes, Sorana; ... mehr

Abstract:
The consumption of wood fuel is markedly increasing in developing and industrialized countries. Known side effects of wood smoke inhalation manifest in proinflammatory signaling, oxidative stress, DNA damage and hence increased cancer risk. In this study, the composition and acute biological impact of emissions of state-of-the-art wood combustion compliances: masonry heater (MH) and pellet boiler (PB) were investigated. Therefore A549 cells were exposed to emission aerosols in an automated air-liquid interface exposure station followed by cytotoxicity, transcriptome and proteome analyses. In parallel, aerosols were subjected to a chemical and physical haracterization. Compared to PB, the MH combustion at the same dilution ratio resulted in a 3-fold higher particle mass concentration (PM2.5) and deposited dose (PB: 27 $\pm$ 2 ng/cm2, MH; 73 $\pm$ 12 ng/cm2). Additionally, the MH aerosol displayed a substantially larger concentration of aldehydes, polycyclic aromatic hydrocarbons (PAH) or oxidized PAH. Gene ontology analysis of transcriptome of A549 cells exposed to MH emissions revealed the activation of proinflammatory response and ... mehr

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Verlagsausgabe §
DOI: 10.5445/IR/1000082224
Veröffentlicht am 15.04.2019
Originalveröffentlichung
DOI: 10.31083/j.jmcm.2018.01.004
Zugehörige Institution(en) am KIT Institut für Toxikologie und Genetik (ITG)
Institut für Technische Chemie (ITC)
Publikationstyp Zeitschriftenaufsatz
Jahr 2018
Sprache Englisch
Identifikator ISSN: 2616-3632
KITopen-ID: 1000082224
HGF-Programm 47.01.01 (POF III, LK 01)
Erschienen in Journal of Molecular and Clinical Medicine
Band 1
Heft 1
Seiten 23-35
Schlagworte Particulate matter; Wood combustion; Log wood; Wood pellets; Masonry heater; Pellet boiler; Air-liquid interface aerosol exposure; Human lung cells; Air-Toxics; Biomarkers; Proteomics; Transcriptomics; Polycyclic aromatic hydrocarbons; Phenolic compounds; Antioxidants; CYP1A1 induction; Soot; Zinc
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