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Originalveröffentlichung
DOI: 10.1364/BOE.9.002511

Cellular in vivo 3D imaging of the cornea by confocal laser scanning microscopy

Bohn, Sebastian; Sperlich, Karsten; Allgeier, Stephan; Bartschat, Andreas; Prakasam, Ruby; Reichert, Klaus-Martin; Stolz, Heinrich; Guthoff, Rudolf; Mikut, Ralf; Köhler, Bernd; Stachs, Oliver

Abstract:
We present an in vivo confocal laser scanning microscopy based method for large 3D reconstruction of the cornea on a cellular level with cropped volume sizes up to 266 x 286 x 396 µm3. The microscope objective used is equipped with a piezo actuator for automated, fast and precise closed-loop focal plane control. Furthermore, we present a novel concave surface contact cap, which significantly reduces eye movements by up to 87%, hence increasing the overlapping image area of the whole stack. This increases the cuboid volume of the generated 3D reconstruction significantly. The possibility to generate oblique sections using isotropic volume stacks opens the window to slit lamp microscopy on a cellular level.


Zugehörige Institution(en) am KIT Institut für Automation und angewandte Informatik (IAI)
Publikationstyp Zeitschriftenaufsatz
Jahr 2018
Sprache Englisch
Identifikator ISSN: 2156-7085, 2156-7085
KITopen ID: 1000082531
HGF-Programm 43.23.02; LK 01
Erschienen in Biomedical optics express
Band 9
Heft 6
Seiten 2511-2525
Vorab online veröffentlicht am 01.05.2018
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