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Cellular in vivo 3D imaging of the cornea by confocal laser scanning microscopy

Bohn, Sebastian; Sperlich, Karsten; Allgeier, Stephan; Bartschat, Andreas; Prakasam, Ruby; Reichert, Klaus-Martin; Stolz, Heinrich; Guthoff, Rudolf; Mikut, Ralf; Köhler, Bernd; Stachs, Oliver

We present an in vivo confocal laser scanning microscopy based method for large 3D reconstruction of the cornea on a cellular level with cropped volume sizes up to 266 x 286 x 396 µm3. The microscope objective used is equipped with a piezo actuator for automated, fast and precise closed-loop focal plane control. Furthermore, we present a novel concave surface contact cap, which significantly reduces eye movements by up to 87%, hence increasing the overlapping image area of the whole stack. This increases the cuboid volume of the generated 3D reconstruction significantly. The possibility to generate oblique sections using isotropic volume stacks opens the window to slit lamp microscopy on a cellular level.

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Verlagsausgabe §
DOI: 10.5445/IR/1000082531
Veröffentlicht am 29.10.2018
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Automation und angewandte Informatik (IAI)
Publikationstyp Zeitschriftenaufsatz
Publikationsmonat/-jahr 06.2018
Sprache Englisch
Identifikator ISSN: 2156-7085, 2156-7085
KITopen-ID: 1000082531
HGF-Programm 43.23.02 (POF III, LK 01) X-Ray Optics
Erschienen in Biomedical optics express
Verlag The Optical Society of America (OSA)
Band 9
Heft 6
Seiten 2511-2525
Vorab online veröffentlicht am 01.05.2018
Schlagwörter Ophthalmology, Medical and biological imaging, Confocal microscopy
Nachgewiesen in Web of Science
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