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3D confocal laser-scanning microscopy for large-area imaging of the corneal subbasal nerve plexus

Allgeier, Stephan ORCID iD icon 1; Bartschat, Andreas 1; Bohn, Sebastian; Peschel, Sabine; Reichert, Klaus-Martin 2; Sperlich, Karsten; Walckling, Marcus; Hagenmeyer, Veit 1; Mikut, Ralf ORCID iD icon 1; Stachs, Oliver; Köhler, Bernd 1
1 Institut für Automation und angewandte Informatik (IAI), Karlsruher Institut für Technologie (KIT)
2 Karlsruher Institut für Technologie (KIT)


The capability of corneal confocal microscopy (CCM) to acquire high-resolution in vivo images of the densely innervated human cornea has gained considerable interest in using this non-invasive technique as an objective diagnostic tool for staging peripheral neuropathies. Morphological alterations of the corneal subbasal nerve plexus (SNP) assessed by CCM have been shown to correlate well with the progression of neuropathic diseases and even predict future-incident neuropathy. Since the field of view of single CCM images is insufficient for reliable characterisation of nerve morphology, several image mosaicking techniques have been developed to facilitate the assessment of the SNP in large-area visualisations. Due to the limited depth of field of confocal microscopy, these approaches are highly sensitive to small deviations of the focus plane from the SNP layer. Our contribution proposes a new automated solution, combining guided eye movements for rapid expansion of the acquired SNP area and axial focus plane oscillations to guarantee complete imaging of the SNP. We present results of a feasibility study using the proposed setup to evaluate different oscillation settings. ... mehr

Verlagsausgabe §
DOI: 10.5445/IR/1000082728
Veröffentlicht am 14.05.2018
DOI: 10.1038/s41598-018-25915-6
Zitationen: 32
Web of Science
Zitationen: 24
Zitationen: 38
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Automation und angewandte Informatik (IAI)
Publikationstyp Zeitschriftenaufsatz
Publikationsmonat/-jahr 12.2018
Sprache Englisch
Identifikator ISSN: 2045-2322
KITopen-ID: 1000082728
HGF-Programm 43.23.02 (POF III, LK 01) X-Ray Optics
Erschienen in Scientific reports
Verlag Nature Research
Band 8
Heft 1
Seiten Article Number 7468
Projektinformation DFG, DFG EIN, MI 1315/5-1
DFG, DFG EIN, KO 5003/1-1
Vorab online veröffentlicht am 10.05.2018
Nachgewiesen in Scopus
Web of Science
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