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Correction: Rational design of a peptide capture agent for CXCL8 based on a model of the CXCL8:CXCR1 complex

Helmer, Dorothea 1; Rink, Ina; Dalton, James A. R.; Brahm, Kevin; Jöst, Marina; Nargang, Tobias M. 1; Blum, Witali; Wadhwani, Parvesh 2; Brenner-Weiss, Gerald 3; Rapp, Bastian E. 1; Giraldo, Jesús; Schmitz, Katja
1 Institut für Mikrostrukturtechnik (IMT), Karlsruher Institut für Technologie (KIT)
2 Institut für Biologische Grenzflächen (IBG), Karlsruher Institut für Technologie (KIT)
3 Institut für Funktionelle Grenzflächen (IFG), Karlsruher Institut für Technologie (KIT)

Abstract:

Correction for ‘Rational design of a peptide capture agent for CXCL8 based on a model of the CXCL8:CXCR1 complex’ by Dorothea Helmer et al., RSC Adv., 2015, 5, 25657–25668.
The authors regret that the original article included some results which were subsequently found to be based on a slightly different peptide sequence than the sequence originally reported. This issue is addressed in the following text, which is an update to the original article.
Upon further investigation of the IL8 capture peptide IL8-RP-Loops introduced in the original article, we found that during synthesis of the intended peptide sequence AKWRMVLRI–Ahx–ADTLMRTQ we had obtained the peptide AKWRMVLRI–Ahx–ADTLMRTE, in which the C-terminal glutamine was replaced with glutamic acid. This was confirmed by high resolution mass spectrometry. The reported high affinity (0.5 ± 0.3 μM) was reproduced by the E-mutant within experimental error (1.1 ± 0.1 μM) but not for the original sequence ending with glutamine. We conclude that all experiments were performed with the peptide AKWRMVLRI–Ahx–ADTLMRTE.
The affected amino acid Q271 was shown to be non-essential for receptor function by Hébert et al.1 So no essential amino acid of the original sequence was omitted in the exchange.
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Verlagsausgabe §
DOI: 10.5445/IR/1000083360
Veröffentlicht am 27.06.2018
Originalveröffentlichung
DOI: 10.1039/C8RA90035C
Scopus
Zitationen: 1
Web of Science
Zitationen: 2
Dimensions
Zitationen: 4
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Biologische Grenzflächen (IBG)
Institut für Funktionelle Grenzflächen (IFG)
Institut für Mikrostrukturtechnik (IMT)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2018
Sprache Englisch
Identifikator ISSN: 2046-2069
urn:nbn:de:swb:90-833604
KITopen-ID: 1000083360
HGF-Programm 47.02.07 (POF III, LK 01) Zellpopul.auf Biofunk.Oberflächen IMT
Erschienen in RSC Advances
Verlag Royal Society of Chemistry (RSC)
Band 8
Heft 30
Seiten 16800–16801
Vorab online veröffentlicht am 09.05.2018
Nachgewiesen in Web of Science
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Scopus
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