Neuro-Information-Systems (NeuroIS) research has become an established approach in the information systems (IS) discipline for investigating and understanding user behavior. Our outlined package with the name brownieR is a freely-available open source R-package for analyzing NeuroIS data (i.e. the combination of physiological and behavioral data). The central purpose of this work is to instruct researchers how brownieR can be used in IS research by providing a practical guide on how to conduct the analysis of bio-physiological data combined with behavioral data (e.g., from the web, experimental tasks, or log files). Further, the article provides an analysis framework and covers the different stages involved in analyzing physiological data.