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Growth and Product Formation of Clostridium ljungdahlii in Presence of Cyanide

Oswald, Florian; Zwick, Michaela; Omar, Ola; Hotz, Ernst N.; Neumann, Anke

Cyanide is a minor constituent of crude syngas whose content depends on the feedstock and gasification procedure. It is a known poison to metal catalysts and inhibits iron-containing enzymes like carbon monoxide dehydrogenase of acetogenic organisms. Therefore, it is considered a component that has to be removed from the gas stream prior to use in chemical synthesis or syngas fermentation. We show that the growth rate and maximum biomass concentration of Clostridium ljungdahlii are unaffected by cyanide at concentrations of up to 1.0 mM with fructose as a carbon source and up to 0.1 mM with syngas as a carbon source. After the culture is adapted to cyanide it shows no growth inhibition. While the difference in growth is an increasing lag-phase with increasing cyanide concentrations, the product spectrum shifts from 97% acetic acid and 3% ethanol at 0 mM cyanide to 20% acetic acid and 80% ethanol at 1.0 mM cyanide for cultures growing on (fructose) and 80% acetic acid and 20% ethanol at 0.1 mM cyanide (syngas).

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Verlagsausgabe §
DOI: 10.5445/IR/1000084116
Veröffentlicht am 02.07.2018
DOI: 10.3389/fmicb.2018.01213
Zitationen: 3
Web of Science
Zitationen: 2
Zugehörige Institution(en) am KIT Institut für Bio- und Lebensmitteltechnik (BLT)
Publikationstyp Zeitschriftenaufsatz
Jahr 2018
Sprache Englisch
Identifikator ISSN: 1664-302X
KITopen-ID: 1000084116
Erschienen in Frontiers in microbiology
Band 9
Seiten Article-Nr.: 1213
Bemerkung zur Veröffentlichung Gefördert durch den KIT-Publikationsfonds
Vorab online veröffentlicht am 13.06.2018
Schlagworte cyanide; Clostridium ljungdahlii; crude syngas; ethanol formation; syngas fermentation; syngas impurities; growth inhibition
Nachgewiesen in Scopus
Web of Science
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