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Transforming plant biology and breeding with CRISPR/Cas9, Cas12 and Cas13

Schindele, Patrick 1; Wolter, Felix 1; Puchta, Holger 1
1 Karlsruher Institut für Technologie (KIT)

Abstract:

Currently, biology is revolutionized by ever growing applications of the CRISPR /Cas system. As discussed in this Review, new avenues have opened up for plant research and breeding by the use of the sequence‐specific DN ases Cas9 and Cas12 (formerly named Cpf1) and, more recently, the RN ase Cas13 (formerly named C2c2). Although double strand break‐induced gene editing based on error‐prone nonhomologous end joining has been applied to obtain new traits, such as powdery mildew resistance in wheat or improved pathogen resistance and increased yield in tomato, improved technologies based on CRISPR /Cas for programmed change in plant genomes via homologous recombination have recently been developed. Cas9‐ and Cas12‐ mediated DNA binding is used to develop tools for many useful applications, such as transcriptional regulation or fluorescence‐based imaging of specific chromosomal loci in plant genomes. Cas13 has recently been applied to degrade mRNA s and combat viral RNA replication. By the possibility to address multiple sequences with different guide RNA s and by the simultaneous use of different Cas proteins in a single cell, we should soon be able to achieve complex changes of plant metabolism in a controlled way.


Verlagsausgabe §
DOI: 10.5445/IR/1000084402
Veröffentlicht am 29.05.2020
Originalveröffentlichung
DOI: 10.1002/1873-3468.13073
Scopus
Zitationen: 62
Web of Science
Zitationen: 50
Dimensions
Zitationen: 77
Cover der Publikation
Zugehörige Institution(en) am KIT Fakultät für Chemie und Biowissenschaften – Botanisches Institut und Botanischer Garten (BOTANIK)
Publikationstyp Zeitschriftenaufsatz
Publikationsmonat/-jahr 06.2018
Sprache Englisch
Identifikator ISSN: 0014-5793
KITopen-ID: 1000084402
Erschienen in FEBS letters
Verlag John Wiley and Sons
Band 592
Heft 12
Seiten 1954–1967
Vorab online veröffentlicht am 10.05.2018
Nachgewiesen in Web of Science
Dimensions
Scopus
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