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Postnatal development and distribution of sympathetic innervation in mouse skeletal muscle

Straka, Tatjana; Vita, V.; Prokshi, K.; Hörner, S. J.; Khan, M. M.; Pirazzini, M.; Williams, M. P. I.; Hafner, M.; Zaglia, T.; Rudolf, Rüdiger

Vertebrate neuromuscular junctions (NMJs) have been conceived as tripartite synapses composed of motor neuron, Schwann cell, and muscle fiber. Recent work has shown the presence of sympathetic neurons in the immediate vicinity of NMJs and experimental and clinical findings suggest that this plays an eminent role in adult NMJ biology. The present study examined the postnatal development and distribution of sympathetic innervation in different muscles using immunofluorescence, confocal microscopy, and Western blot. This demonstrates the proximity of sympathetic neurons in diaphragm, extensor digitorum longus, tibialis anterior, soleus, and levator auris longus muscles. In extensor digitorum longus muscle, sympathetic innervation of NMJs was quantified from perinatal to adult stage and found to increase up to two months of age. In diaphragm muscle, an extensive network of sympathetic neurons was prominent along the characteristic central synapse band. In summary, these data demonstrate that an elaborate sympathetic innervation is present in several mouse skeletal muscles and that this is often next to NMJs. Although the presence of sympathetic neurons at the perisynaptic region of NMJs increased during postnatal development, many synapses were already close to sympathetic neurons at birth. ... mehr

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Verlagsausgabe §
DOI: 10.5445/IR/1000084708
Veröffentlicht am 23.07.2018
DOI: 10.3390/ijms19071935
Zitationen: 12
Web of Science
Zitationen: 11
Zugehörige Institution(en) am KIT Institut für Toxikologie und Genetik (ITG)
Publikationstyp Zeitschriftenaufsatz
Jahr 2018
Sprache Englisch
Identifikator ISSN: 1422-0067, 1661-6596
KITopen-ID: 1000084708
HGF-Programm 47.01.01 (POF III, LK 01)
Erschienen in International journal of molecular sciences
Band 19
Heft 7
Seiten 1935
Schlagworte endplate; neuromuscular junction; sympathetic neuron; tyrosine hydroxylase; neuropeptide Y
Nachgewiesen in Scopus
Web of Science
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