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Site-specific anchoring aptamer C2NP on DNA origami nanostructures for cancer treatment

Sun, P. 1; Zhang, N.; Tang, Y.; Yang, Y.; Zhou, J.; Zhao, Y.
1 Institut für Biologische Grenzflächen (IBG), Karlsruher Institut für Technologie (KIT)

Because of the remarkable features, including biocompatibility and biodegradability, DNA origami nanostructures have drawn much attention as ideal carriers for drug delivery. However, the cellular uptake of DNA origami nanostructures was a passive targeting process, resulting in limited therapeutic effect. To address this problem, we anchored the aptamer C2NP (Apt) on rectangular DNA origami nanostructures (RE) to enhance the tumor targeting properties and anticancer effects of doxorubicin (DOX). Apt was anchored onto RE with low or high density (RE-4Apt, RE-16Apt), followed by incubation with DOX to obtain DOX@RE-4Apt and DOX@RE-16Apt. The results showed that DOX@RE-4Apt and DOX@RE-16Apt exhibited excellent biocompatibility and targeting ability, as well as a synergic biological effect with chemotherapy on cancer therapy. More importantly, after conjugation with RE, the bioactivity of Apt was significantly increased. These results revealed that Apt anchored DNA nanostructures not only are potential carriers for precise therapy, but also supply a strategy to enhance the bioactivity of aptamers.

Verlagsausgabe §
DOI: 10.5445/IR/1000085221
Veröffentlicht am 04.09.2018
DOI: 10.1039/c8ra04589e
Zitationen: 7
Web of Science
Zitationen: 7
Zitationen: 8
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Biologische Grenzflächen (IBG)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2018
Sprache Englisch
Identifikator ISSN: 2046-2069
KITopen-ID: 1000085221
HGF-Programm 47.02.01 (POF III, LK 01) Zellpopul.auf Biofunk.Oberflächen IBG-1
Erschienen in RSC Advances
Verlag Royal Society of Chemistry (RSC)
Band 8
Heft 46
Seiten 26300-26308
Vorab online veröffentlicht am 23.07.2018
Nachgewiesen in Scopus
Web of Science
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