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Verlagsausgabe
DOI: 10.5445/IR/1000085876
Veröffentlicht am 24.09.2018
Originalveröffentlichung
DOI: 10.1186/s13046-018-0872-6
Scopus
Zitationen: 2
Web of Science
Zitationen: 3

Liver cancer cell lines distinctly mimic the metabolic gene expression pattern of the corresponding human tumours

Nwosu, Z. C.; Battello, N.; Rothley, M.; Piorońska, W.; Sitek, B.; Ebert, M. P.; Hofmann, U.; Sleeman, J.; Wölfl, S.; Meyer, C.; Megger, D. A.; Dooley, S.

Abstract:
Background: Although metabolism is profoundly altered in human liver cancer, the extent to which experimental models, e.g. cell lines, mimic those alterations is unresolved. Here, we aimed to determine the resemblance of hepatocellular carcinoma (HCC) cell lines to human liver tumours, specifically in the expression of deregulated metabolic targets in clinical tissue samples.
Methods: We compared the overall gene expression profile of poorly-differentiated (HLE, HLF, SNU-449) to well-differentiated (HUH7, HEPG2, HEP3B) HCC cell lines in three publicly available microarray datasets. Three thousand and eighty-five differentially expressed genes in ≥2 datasets (P < 0.05) were used for pathway enrichment and gene ontology (GO) analyses. Further, we compared the topmost gene expression, pathways, and GO from poorly differentiated cell lines to the pattern from four human HCC datasets (623 tumour tissues). In well- versus poorly differentiated cell lines, and in representative models HLE and HUH7 cells, we specifically assessed the expression pattern of 634 consistently deregulated metabolic genes in human HCC. These data were complem ... mehr


Zugehörige Institution(en) am KIT Institut für Toxikologie und Genetik (ITG)
Publikationstyp Zeitschriftenaufsatz
Jahr 2018
Sprache Englisch
Identifikator ISSN: 0392-9078, 1756-9966
URN: urn:nbn:de:swb:90-858762
KITopen-ID: 1000085876
HGF-Programm 47.01.01 (POF III, LK 01)
Erschienen in Journal of experimental & clinical cancer research
Band 37
Heft 1
Seiten Art. Nr.: 211
Schlagworte Omics, Tumour metabolism, Well-differentiated, Poorly differentiated, Metabolic vulnerability, Selective therapy, Biomarkers, MAPK/Ras signaling, Hepatocellular carcinoma
Nachgewiesen in Scopus
Web of Science
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