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Verlagsausgabe
DOI: 10.5445/IR/1000086105
Veröffentlicht am 01.10.2018
Originalveröffentlichung
DOI: 10.1038/s41598-018-29749-0

Comparative analysis of surface coating properties of five hydrophobins from Aspergillus nidulans and Trichoderma reseei

Winandy, Lex; Hilpert, Felix; Schlebusch, Oleksandra; Fischer, Reinhard

Abstract:
Fungal hydrophobins are small amphiphilic proteins that self-assemble into monolayers on hydrophobic:hydrophilic interfaces and can be used for surface coatings. Because e.g. Aspergillus nidulans contains six different hydrophobins, it is likely that they have different properties and are used for different “applications” in the fungus. We established a method for recombinant production of different class hydrophobins in Escherichia coli. We produced DewA, DewC, DewD, DewE from A. nidulans and HFBI from Trichoderma reesei and compared surface coating properties of these hydrophobins. All tested proteins formed coatings on glass, strongly increasing the hydrophobicity of the surface, and showed emulsion-stabilizing properties. But whereas the typical class I hydrophobin DewA formed the most stable coating on glass, the intermediate class hydrophobins DewE and DewD were more effective in stabilization of oil:water emulsions. This work gives insights into correlations between structural characteristics of hydrophobins and their behaviour as surface binding agents. It could help with the clarification of their biological functions and l ... mehr


Zugehörige Institution(en) am KIT Institut für Angewandte Biowissenschaften (IAB)
Publikationstyp Zeitschriftenaufsatz
Jahr 2018
Sprache Englisch
Identifikator ISSN: 2045-2322
URN: urn:nbn:de:swb:90-861057
KITopen-ID: 1000086105
Erschienen in Scientific reports
Band 8
Heft 1
Seiten Article: 12033
Bemerkung zur Veröffentlichung Gefördert durch den KIT-Publikationsfonds
Vorab online veröffentlicht am 13.08.2018
Nachgewiesen in Scopus
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