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Enantiomer discrimination in β-phenylalanine degradation by a newly isolated Paraburkholderia strain BS115 and type strain PsJN

Buß, Oliver 1; Dold, Sarah-Marie 1; Obermeier, Pascal 1; Litty, Dennis 1; Muller, Delphine 1; Grüninger, Jens 1; Rudat, Jens 1
1 Institut für Bio- und Lebensmitteltechnik (BLT), Karlsruher Institut für Technologie (KIT)


Despite their key role in numerous natural compounds, β-amino acids have rarely been studied as substrates for microbial degradation. Fermentation of the newly isolated Paraburkholderia strain BS115 and the type strain P. phytofirmans PsJN with β-phenylalanine (β-PA) as sole nitrogen source revealed (S)-selective transamination of β-PA to the corresponding β-keto acid by both strains, accompanied by substantial formation of acetophenone (AP) from spontaneous decarboxylation of the emerging β-keto acid. While the PsJN culture became stationary after entire (S)-β-PA consumption, BS115 showed further growth at a considerably slower rate, consuming (R)-β-PA without generation of AP which points to a different degradation mechanism for this enantiomer. This is the first report on degradation of both enantiomers of any β-amino acid by one single bacterial strain.

Verlagsausgabe §
DOI: 10.5445/IR/1000086228
Veröffentlicht am 05.10.2018
DOI: 10.1186/s13568-018-0676-2
Zitationen: 2
Web of Science
Zitationen: 2
Zitationen: 2
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Bio- und Lebensmitteltechnik (BLT)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2018
Sprache Englisch
Identifikator ISSN: 2191-0855
KITopen-ID: 1000086228
Erschienen in AMB express
Verlag SpringerOpen
Band 8
Heft 1
Seiten Article: 149
Bemerkung zur Veröffentlichung Gefördert durch den KIT-Publikationsfonds
Schlagwörter β-Phenylalanine, ω-Transaminase, Kinetic resolution, Chiral resolution, Paraburkholderia sp., Fermentation, Acetophenone degradation
Nachgewiesen in Scopus
Web of Science
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