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DOI: 10.5445/IR/1000086406
Veröffentlicht am 11.10.2018

Detailed Structural Characterization of Arabinans and Galactans of 14 Apple Cultivars Before and After Cold Storage

Wefers, Daniel; Flörchinger, Ramona; Bunzel, Mirko

Abstract:
Physiological and textural properties of apples are greatly influenced by both cultivar and structural composition of their pectic polysaccharides. In previous studies, it was demonstrated that neutral pectic side chains (arabinans and galactans) play a major role during fruit development and postharvest processes. However, these complex polymers have a high structural heterogeneity, and some structural elements such as side chain substituents and substitution of neighboring residues cannot be analyzed by using conventional analytical methods. Therefore, fine structures of arabinans and galactans were analyzed in 14 apple cultivars before and after storage. Besides conventional methods such as methylation analysis, profiling approaches based on enzymatic cleavage were applied to obtain detailed information on the neutral side chains of pectins. Structurally different, highly branched arabinans and linear β-1,4-linked galactans were detected in all cultivars. By using enzymatic profiling approaches, rare structural elements such as β-arabinofuranose and α-arabinopyranose residues were detected. In addition, the combination of all met ... mehr


Zugehörige Institution(en) am KIT Institut für Angewandte Biowissenschaften (IAB)
Publikationstyp Zeitschriftenaufsatz
Jahr 2018
Sprache Englisch
Identifikator ISSN: 1664-462X
URN: urn:nbn:de:swb:90-864067
KITopen-ID: 1000086406
Erschienen in Frontiers in plant science
Band 9
Seiten Article: 1451
Bemerkung zur Veröffentlichung Gefördert durch den KIT-Publikationsfonds
Vorab online veröffentlicht am 02.10.2018
Schlagworte pectins; arabinans, galactans; dietary fiber; cell wall; NMR spectroscopy; HPAEC-PAD; enzymatic hydrolysis
Nachgewiesen in Web of Science
Scopus
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