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DOI: 10.5445/IR/1000086554
Veröffentlicht am 15.10.2018
DOI: 10.1371/journal.pgen.1007674

The topoisomerase 3α zinc-finger domain T1 of Arabidopsis thaliana is required for targeting the enzyme activity to Holliday junction-like DNA repair intermediates

Dorn, Annika; Röhrig, Sarah; Papp, Kristin; Schröpfer, Susan; Hartung, Frank; Knoll, Alexander; Puchta, Holger

Topoisomerase 3α, a class I topoisomerase, consists of a TOPRIM domain, an active centre and a variable number of zinc-finger domains (ZFDs) at the C-terminus, in multicellular organisms. Whereas the functions of the TOPRIM domain and the active centre are known, the specific role of the ZFDs is still obscure. In contrast to mammals where a knockout of TOP3α leads to lethality, we found that CRISPR/Cas induced mutants in Arabidopsis are viable but show growth retardation and meiotic defects, which can be reversed by the expression of the complete protein. However, complementation with AtTOP3α missing either the TOPRIM-domain or carrying a mutation of the catalytic tyrosine of the active centre leads to embryo lethality. Surprisingly, this phenotype can be overcome by the simultaneous removal of the ZFDs from the protein. In combination with a mutation of the nuclease AtMUS81, the TOP3α knockout proved to be also embryo lethal. Here, expression of TOP3α without ZFDs, and in particular without the conserved ZFD T1, leads to only a partly complementation in root growth—in contrast to the complete protein, that restores root length to m ... mehr

Zugehörige Institution(en) am KIT Botanisches Institut und Botanischer Garten (BOTANIK)
Publikationstyp Zeitschriftenaufsatz
Jahr 2018
Sprache Englisch
Identifikator ISSN: 1553-7404
URN: urn:nbn:de:swb:90-865540
KITopen ID: 1000086554
Erschienen in PLoS Genetics
Band 14
Heft 9
Seiten e1007674
Bemerkung zur Veröffentlichung Gefördert durch den KIT-Publikationsfonds
Vorab online veröffentlicht am 17.09.2018
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