Rapid manufacturing of micro- and nano-structured polymers was achieved by laser-assisted hot embossing technique. Metallic
mold surfaces containing fabricated nanostructures have been replicated using thermoplastics such as polystyrene (PS) and cyclic
olefin copolymer (COC). The applied nano-sized structures were fabricated on stainless steel using ultrafast laser radiation to
generate Laser-Induced Periodic Surface Structures (LIPSS) for control of wettability and bio-compatibility. Furthermore,
electroformed nickel shims containing nanostructures were replicated using COC for subsequent surface-enhanced Raman
spectroscopy (SERS) measurements. It was demonstrated that laser-assisted moulding of thermoplastics offers a cost-efficient and
flexible technique for the production of functional polymeric surfaces with nano-sized features.