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DOI: 10.5445/IR/1000087395
Veröffentlicht am 12.11.2018

Quantitative morphometric analysis of adult teleost fish by X-ray computed tomography

Weinhardt, Venera; Shkarin, Roman; Wernet, Tobias; Wittbrodt, Joachim; Baumbach, Tilo; Loosli, Felix

Abstract:
Vertebrate models provide indispensable paradigms to study development and disease. Their analysis requires a quantitative morphometric study of the body, organs and tissues. This is often impeded by pigmentation and sample size. X-ray micro-computed tomography (micro-CT) allows high-resolution volumetric tissue analysis, largely independent of sample size and transparency to visual light. Importantly, micro-CT data are inherently quantitative. We report a complete pipeline of high-throughput 3D data acquisition and image analysis, including tissue preparation and contrast enhancement for micro-CT imaging down to cellular resolution, automated data processing and organ or tissue segmentation that is applicable to comparative 3D morphometrics of small vertebrates. Applied to medaka fish, we first create an annotated anatomical atlas of the entire body, including inner organs as a quantitative morphological description of an adult individual. This atlas serves as a reference model for comparative studies. Using isogenic medaka strains we show that comparative 3D morphometrics of individuals permits identification of quantitative strai ... mehr


Zugehörige Institution(en) am KIT Institut für Toxikologie und Genetik (ITG)
Laboratorium für Applikationen der Synchrotronstrahlung (LAS)
Institut für Automation und angewandte Informatik (IAI)
Institut für Photonenforschung und Synchrotronstrahlung (IPS)
Publikationstyp Zeitschriftenaufsatz
Jahr 2018
Sprache Englisch
Identifikator ISSN: 2045-2322
URN: urn:nbn:de:swb:90-873953
KITopen ID: 1000087395
HGF-Programm 47.01.01; LK 01
Erschienen in Scientific reports
Band 8
Heft 1
Seiten Art. Nr.: 16531
Bemerkung zur Veröffentlichung Gefördert durch den KIT-Publikationsfonds
Vorab online veröffentlicht am 08.11.2018
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