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Structural snapshot of a bacterial phytochrome in its functional intermediate state

Schmidt, Andrea; Sauthof, Luisa; Szczepek, Michal; Lopez, Maria Fernandez; Escobar, Francisco Velazquez; Qureshi, Bilal M.; Michael, Norbert; Buhrke, David; Stevens, Tammo; Kwiatkowski, Dennis; Stetten, David von; Mroginski, Maria Andrea; Krauß, Norbert 1; Lamparter, Tilman 1; Hildebrandt, Peter; Scheerer, Patrick
1 Karlsruher Institut für Technologie (KIT)


Phytochromes are modular photoreceptors of plants, bacteria and fungi that use light as a source of information to regulate fundamental physiological processes. Interconversion between the active and inactive states is accomplished by a photoinduced reaction sequence which couples the sensor with the output module. However, the underlying molecular mechanism is yet not fully understood due to the lack of structural data of functionally relevant intermediate states. Here we report the crystal structure of a Meta-F intermediate state of an Agp2 variant from Agrobacterium fabrum. This intermediate, the identity of which was verified by resonance Raman spectroscopy, was formed by irradiation of the parent Pfr state and displays significant reorientations of almost all amino acids surrounding the chromophore. Structural comparisons allow identifying structural motifs that might serve as conformational switch for initiating the functional secondary structure change that is linked to the (de-)activation of these photoreceptors.

Verlagsausgabe §
DOI: 10.5445/IR/1000088220
Veröffentlicht am 03.12.2018
DOI: 10.1038/s41467-018-07392-7
Zitationen: 56
Web of Science
Zitationen: 44
Zitationen: 60
Cover der Publikation
Zugehörige Institution(en) am KIT Fakultät für Chemie und Biowissenschaften – Botanisches Institut und Botanischer Garten (BOTANIK)
Publikationstyp Zeitschriftenaufsatz
Publikationsmonat/-jahr 11.2018
Sprache Englisch
Identifikator ISSN: 2041-1723
KITopen-ID: 1000088220
Erschienen in Nature Communications
Verlag Nature Research
Band 9
Heft 1
Seiten 4912
Vorab online veröffentlicht am 21.11.2018
Nachgewiesen in Web of Science
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