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Microstructure, local viscoelasticity and cell culture suitability of 3D hybrid HA/collagen scaffolds

Roether, Johanna; Bertels, Sarah; Oelschlaeger, Claude; Bastmeyer, Martin; Willenbacher, Norbert

As mechanical properties of cell culture substrates matter, methods for mechanical characterization of scaffolds on a relevant length scale are required. We used multiple particle tracking microrheology to close the gap between elasticity determined from bulk measurements and elastic properties sensed by cells. Structure and elasticity of macroporous, three-dimensional cryogel scaffolds from mixtures of hyaluronic acid (HA) and collagen (Coll) were characterized. Both one-component gels formed homogeneous networks, whereas hybrid gels were heterogeneous in terms of elasticity. Most strikingly, local elastic moduli were significantly lower than bulk moduli presumably due to non-equilibrium chain conformations between crosslinks. This was more pronounced in Coll and hybrid gels than in pure HA gels. Local elastic moduli were similar for all gels, irrespective of their different swelling ratio and bulk moduli. Fibroblast cell culture proved the biocompatibility of all investigated compositions. Coll containing gels enabled cell migration, adhesion and proliferation inside the gels.

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Verlagsausgabe §
DOI: 10.5445/IR/1000089039
Veröffentlicht am 04.01.2019
Zugehörige Institution(en) am KIT Institut für Mechanische Verfahrenstechnik und Mechanik (MVM)
Zoologisches Institut (ZOO)
Publikationstyp Zeitschriftenaufsatz
Jahr 2018
Sprache Englisch
Identifikator ISSN: 1932-6203
KITopen-ID: 1000089039
Erschienen in PLOS ONE
Band 13
Heft 12
Seiten Article: e0207397
Bemerkung zur Veröffentlichung Gefördert durch den KIT-Publikationsfonds
Vorab online veröffentlicht am 19.12.2018
Nachgewiesen in Web of Science
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