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DOI: 10.5445/IR/1000089042
Veröffentlicht am 04.01.2019

A Compartmented Flow Microreactor System for Automated Optimization of Bioprocesses Applying Immobilized Enzymes

Heinzler, Raphael; Hübner, Jonas; Fischöder, Thomas; Elling, Lothar; Franzreb, Matthias

Abstract:
In the course of their development, industrial biocatalysis processes have to be optimized in small-scale, e. g., within microfluidic bioreactors. Recently, we introduced a novel microfluidic reactor device, which can handle defined reaction compartments of up to 250 μL in combination with magnetic micro carriers. By transferring the magnetic carriers between subsequent compartments of differing compositions, small scale synthesis, and bioanalytical assays can be conducted. In the current work, this device is modified and extended to broaden its application range to the screening and optimization of bioprocesses applying immobilized enzymes. Besides scaling the maximum compartment volume up to 3 mL, a temperature control module, as well as a focused infrared spot were integrated. By adjusting the pump rate, compartment volumes can be accurately dosed with an error <5% and adjusted to the requested temperature within less than a minute. For demonstration of bioprocess parameter optimization within such compartments, the influence of pH, temperature, substrate concentration, and enzyme carrier loading was automatically screened for ... mehr


Zugehörige Institution(en) am KIT Institut für Funktionelle Grenzflächen (IFG)
Publikationstyp Zeitschriftenaufsatz
Jahr 2018
Sprache Englisch
Identifikator ISSN: 2296-4185
URN: urn:nbn:de:swb:90-890423
KITopen-ID: 1000089042
HGF-Programm 47.02.06 (POF III, LK 01)
Erschienen in Frontiers in Bioengineering and Biotechnology
Band 6
Seiten Article: 189
Bemerkung zur Veröffentlichung Gefördert durch den KIT-Publikationsfonds
Vorab online veröffentlicht am 04.12.2018
Schlagworte bioreactor; magnetic particles; process development and optimization; immobilized enzymatic reactor; automated microreaction system
Nachgewiesen in Web of Science
Scopus
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